W C Venier

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OBJECTIVE To compare the survival rate of frozen-thawed embryos with perforated zonae from microsurgical fertilization (subzonal insemination) (SUZI) with that of embryos with intact zonae. DESIGN Thirty-eight embryos resulting from microsurgical fertilization by SUZI were cryopreserved in 16 patient cycles. Within the same period, 140 zonae-intact(More)
Three methods were used to prepare spermatozoa for subzonal injection into mature oocytes. In method A, the washed sperm suspension was incubated for 18 h in modified T6 culture medium. Method B consisted of incubating the sperm suspension for 6 h in regular T6 culture medium. In method C, the sperm suspension was incubated for 6 h in regular T6 culture(More)
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