Vytautas Klausa

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Bacteriophages vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20, showing an unusual low-temperature plating profile and producing constantly growing plaques, were isolated from aquatic environments of Lithuania. Although vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20 resembled phage T4 both in their genome size and in their major structural protein (gp23) pattern,(More)
Despite the fact that multidrug-resistant Klebsiella sp. strains emerge rapidly (Xu J, et al., Adv. Mater. Res. 268-270:1954-1956, 2011) and bacteriophages have been reported to be useful in controlling these bacteria (Kumari S, Harjai K, Chhibber S, J. Med. Microbiol. 60:205-210, 2011), the complete genome sequences of only five Klebsiella phages (four(More)
The complete genome sequence of the T4-related low-temperature Escherichia coli bacteriophage vB_EcoM-VR7 was determined. The genome sequence is 169,285 bp long, with a G+C content of 40.3%. Overall, 95% of the phage genome is coding. It encodes 293 putative protein-encoding open reading frames (ORFs) and tRNAMet. More than half (59%) of the genomic DNA(More)
A proposed new genus of the family Myoviridae, “rV5-like viruses”, includes two lytic bacteriophages: Escherichia coli O157: H7-specific bacteriophage rV5 and Salmonella phage PVP-SE1. Here, we present basic properties and genomic characterization of a novel rV5-like phage, vB_EcoM_FV3, which infects E. coli K-12-derived laboratory strains and replicates at(More)
Secondary structure of the mRNA in the translational initiation region is an important determinant of translation efficiency. However, the secondary structures that enhance or facilitate translation initiation are rare. We have previously proposed that such structure may exist in the case of bacteriophage T4 gene 25 translational initiation region, which(More)
Bacteriophage T4 middle-mode transcription requires Escherichia coli RNA polymerase, phage-encoded transcriptional activator MotA and co-activator AsiA that form a complex at a middle promoter DNA. T4 middle promoters have been defined by a consensus sequence deduced from the list of 14 middle promoters identified in earlier studies. To date, 33 middle(More)
The bacterial strains with recombinant plasmids constructed on the basis of vector plasmid pSCC31 and containing BglII fragment of bacteriophage T4 DNA with genes 25-29 have been used in this study. Restriction analysis and subcloning demonstrated that in the case of the recombinant plasmid pRL705, the phage DNA fragment had right orientation for phage late(More)
The dependence of multiplication of genes 26 and 33 amber mutants of T4D phage in a non-permissive host on the temperature in the range of 25-41 degrees C has been studied. The secondary mutations observed in the genome of gene 26 mutants amS105 and amNG114 were excluded by crossing these mutants with the wild type phage. It was established that(More)
We used bacterial strains with two recombinant plasmids carrying BglII DNA fragment with T4 genes 25-29 in different orientations. In the case of the plasmid pRL705 phage DNA fragment had correct orientation for transcription of T4 late genes, and in the plasmid pRL707 it has the opposite one. Transcription from the PL promoter of the plasmid pRL705 led to(More)
The nucleotide sequence of the 907-bp XbaI-EcoRV T4 DNA fragment containing gene 51 is presented. The sequence of gene 51 predicts a 249 amino acid peptide with an M(r) of 29387 and a pl of 6.34. We have cloned and overexpressed this gene in the T7 RNA polymerase system. The observed molecular mass of gp51 is in agreement with the sequence data. We also(More)
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