Vyacheslav N. Chesnokov

We don’t have enough information about this author to calculate their statistics. If you think this is an error let us know.
Learn More
In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used(More)
Using immunochemical and, subsequently, autoradiographic methods, the authors have shown that after the injection of the total fraction of polyribosomes isolated from the rat liver in the Acetabularia cytoplasm a protein interacting with the antiserum against rat thyrosine aminotransferase is synthesized. Prospects of using Acetabularia as a test-system for(More)
The data concerning the cycloheximide influence on individual mRNA content, transcription rate and chromatin structure in mammalian cells are reviewed. The putative mechanism of cycloheximide effect is explained as being due to the existence of short-living regulatory proteins. It is concluded that cycloheximide is a helpful tool in the study of regulation(More)
The matrix activity of phage T7 DNA and the restriction fragment of phage T7 DNA containing gene 0,3 with a promoter was studied with respect to RNA and protein syntheses in a cell-free transcription-translation system from E. coli. A comparison of the protein products of cell-free synthesis was carried out, using polyacrylamide gel electrophoresis and a(More)
Treatment of Wistar rats with a single dose of hydrocortisone acetate resulted in a transient induction of tyrosine aminotransferase (TAT) in the liver. TAT activity and the level of TAT mRNA increased 4-6 h after addition of hydrocortisone acetate (induction phase) and declined thereafter (deinduction phase). TAT activity and TAT mRNA failed to respond to(More)