Vlastimila Špaková

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A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for(More)
Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in(More)
Phytotoxicity of acyclic nucleoside phosphonates (ANPs), namely (R)-PMPA, (S)-PMPA, (S)-PMPG, (S)-HPMPO-DAPy, N 6-cPr-PMEDAP, (S)-HPMPC, PMEDAP, (S)-FPMPA, N 6-furfuryl-PMEA, (S)-HPMPA, PMEG, (R)-PMPDAP, PMEO-DAPy, (R,S)-2′-CF3-PMEDAP, (R)-8-PMP-8-azaA, and (R)-PMP-3-deazaA, comprising potential antiviral agents against plant DNA viruses, was tested in(More)
We collected samples from black, red and white currants showing symptoms of blackcurrant reversion disease (BRD) and full blossom disease (FBD), cultivated in the Czech Republic. Blackcurrant reversion virus (BRV) was detected in all symptomatic plants. After amplification, a substantial part of the 3′ non-translated region (3′-NTR) of RNA2 of 15 new(More)
The antiviral effect of the acyclic nucleoside phosphonate tenofovir (R)-PMPA on double-stranded DNA Cauliflower mosaic virus (CaMV) in Brassica pekinensis plants grown in vitro on liquid medium was evaluated. Double antibody sandwich ELISA and PCR were used for relative quantification of viral protein and detecting nucleic acid in plants. (R)-PMPA at(More)
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