Vladimir Samoylov

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Soybean [Glycine max (L.) Merrill] somatic embryos of the cultivar Jack underwent histodifferentiation in liquid Murashige and Skoog (MS) medium with 3% maltose, or according to the standard published procedure employing solidified MS media, permitting the recovery of an average of 8.1 and 3.9 embryos/mg of embryogenic tissue, respectively. Cotyledon-stage(More)
To improve proliferation of soybean cultures in liquid medium, the effects of sucrose; total inorganic nitrogen; content of No3 −, NH4 +, Ca2+, PO4 3−, K+; NH4 +/NO3 − ratio; and medium osmotic pressure were studied using cv. Jack. Sucrose concentration, osmotic pressure, total nitrogen content, and ammonium to nitrate ratio were found to be the major(More)
Significant amounts of cell wall degrading (CWD) enzymes are required to degrade lignocellulosic biomass into its component sugars. One strategy for reducing exogenous enzyme production requirements is to produce the CWD enzymes in planta. For this work, various CWD enzymes were expressed in maize (Zea mays). Following growth and dry down of the plants,(More)
Morphology, the extent of elimination of donor chromosomes and the organelle composition of highly asymmetric somatic hybrid plants between a interspecific tomato hybrid Lycopersicon esculentum x L. pennellii (EP) as donor and a Solarium melongena, eggplant (E), recipient, were studied. Morphologically, the somatic hybrids most resemble eggplant but, due to(More)
Plant cellulosic biomass is an abundant, low-cost feedstock for producing biofuels and chemicals. Expressing cell wall-degrading (CWD) enzymes (e.g. xylanases) in plant feedstocks could reduce the amount of enzymes required for feedstock pretreatment and hydrolysis during bioprocessing to release soluble sugars. However, in planta expression of xylanases(More)
Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma-irradiated mesophyll protoplasts of the kanamycin-resistant (KmR+) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with mesophyll protoplasts of Solanum melongena (eggplant, E). Elimination of the EP chromosomes was obtained by irradiating the donor genome(More)
In planta expression of cell wall degrading enzymes is a promising approach for developing optimized biomass feedstocks that enable low-cost cellulosic biofuels production. Transgenic plants could serve as either an enzyme source for the hydrolysis of pretreated biomass or as the primary biomass feedstock in an autohydrolysis process. In this study, two(More)
The paper presents a model, multi-agent architecture, implementation approach and software prototype of a multi-agent system for autonomous air traffic control within airport airspace capable of automatic detection of potential violations of safety policies by individual aircraft and consequent incident management. It features a model facilitating practical(More)
Ever increasing use of heterogeneous networks including mobile devices and ad-hoc sensor networks signifies the role of such information system properties as openness, autonomy, cooperation, coordination, etc. Agent-based service-oriented Peer-to-Peer (P2P) architecture provides attractive (if not single) design and implementation paradigm for such systems.(More)
Regeneration of cell walls in protoplasts was investigated using light- and electronmicroscopic methods. The protoplasts were isolated from mesophyll of Solanum tuberosum leaves and were cultivated on the horizontal low rotating clinostat (2 rpm) and in control for 10 days. Using a fluorescent method (with Calcofluor white) it was demonstrated that(More)