Vladimir Ratner

Learn More
The phenomenon of transposition induction by heavy heat shock (HHS) was studied. Males of a Drosophila isogenic line with a mutation in the major gene radius incompletus (ri) were treated by HHS (37 degrees C for 1 h followed by 4 degrees C for 1 h, with the cycle repeated three times) and crossed to untreated females of the same line. The males were(More)
Males of a Drosophila melanogaster isogenic line with a mutation of the major gene for radius incompletus (ri) were treated by standard light heat shock (37 degrees C for 90 min) and by heavy heat shock (transfer of males from 37 degrees C for 2 hr to 4 degrees C for 1 hr and back; this procedure was repeated three times). In the F1 generation of treated(More)
A polygenic system of expression of the quantitative character radius incompletus was shown to be sensitive to external and physiological stresses: heat shock, gamma-irradiation, isogenization, etc. This stress response involved mobilization of retrotransposons. Heavy heat shock induced transpositions of Dm412 and B104 in three and one isogenic lines,(More)
The dose dependence of the rate of γ-induced transpositions and consequent dynamics of the MGE 412pattern after γ-irradiation were investigated in isogenic line 49 in generations F1, F12, F140, and F170. It was shown that the results on dose dependence of transpositions was very similar with the corresponding results of the classic works by(More)
A variety of biophysical methods used to study proteins requires protein modification using conjugated molecular probes. Cysteine is the main residue that can be modified without the risk of altering other residues in the protein chain. It is possible to label several cysteines in a protein using highly selective labeling reactions, if the cysteines react(More)
In an isogenic line of Drosophila, transpositions of mobile genetic elements (MGE) Dm412 were induced by gamma-radiation at doses of 300, 800, and 1300 R. The rates of induced transpositions were (for each dose, respectively) 3.9 x 10(-3), 1.0 x 10(-2), and 1.87 x 10(-2) events per occupied site per haploid genome of the isogenic line per generation. Thus,(More)
Induction of transpositions by heavy heat shock (HHS) was demonstrated for the copia-like mobile genetic element B104. The probability of transposition induction lambda HHS was equal to 8.1 x 10(-2) per site per spermatozoon per spermatozoon per generation, which is an order of magnitude higher than the upper limit of spontaneous transposition probability(More)
Localization patterns of mobile genetic element (MGE) Dm412 were compared in chromosomes of ten isogenic lines and a heterogeneous control line riC of Drosophila. Isogenization was shown to induce MGE transpositions and excisions. Rates of induced transpositions and excisions were, respectively, 0.35 and 0.13 per site per haploid genome per isogenization,(More)
Alpha-synuclein is regarded as a presynaptic protein, which may play an important role in neuronal plasticity. However, the actual physiological function of this protein is not completely clear. Abnormal accumulation of fibrillar alpha-synuclein in Lewy bodies, as well as mutations in the alpha-synuclein gene identified in the familial forms of Parkinson's(More)