Vladimir Orbović

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Citrus canker, caused by the bacterial pathogen Xanthomonas citri subsp. citri (Xcc), is a serious leaf and fruit spotting disease affecting many important citrus cultivars including grapefruit and certain sweet oranges. Currently, efficacious and economical disease control measures for highly susceptible citrus cultivars are lacking. Development of(More)
We have previously shown that endoreduplication levels in hypocotyls of Arabidopsis thaliana (L.) Heynh. are under negative control of phytochromes. In this study, the hormonal regulation of this process was analysed using a collection of A. thaliana mutants. The results show that two hormones in particular, gibberellin (GA) and ethylene, play distinct(More)
Copper (Cu)-based fungicidal sprays are widely used on many crops although Cu sprays can be phytotoxic under some conditions. The mechanism of phytotoxicity is poorly understood but must involve toxic levels of Cu penetrating plant tissues. We studied the effect of different adjuvants on the deposition pattern of droplets and penetration of Cu (in Kocide(More)
Competence for phototropic (PT) and gravitropic (GT) bending by potato plantlets grown in vitro manifests regular daily changes indicating possible involvement of circadian regulation. Unilateral stimulation of plantlets with blue light at dawn resulted in moderate PT response regarding both attained curvature and long lag phase. The PT response was the(More)
A possible role of arabinogalactan proteins in control of shoot regeneration from stem explants of two citrus cultivars, Carrizo citrange and ‘Duncan’ grapefruit, was investigated. Treatment of explants with (β-d-Glc)3 Yariv phenylglycoside, able to bind specifically to AGPs, led to a decrease of cumulative regeneration potential of both Carrizo citrange(More)
Citrus canker caused by Xanthomonas citri subspecies citri (Xcc) is a severe disease for most commercial citrus cultivars and responsible for significant economic losses worldwide. Generating canker-resistant citrus varieties will provide an efficient and sustainable solution to control citrus canker. Here, we report our progress in generating(More)
Etiolated and light-grown plantlets obtained from potato shoot cultures were shown to perform vigorous tropic movements. Unilateral blue irradiation actively induced phototropic curvature of the shoots toward the light source, although etiolated plantlets required ten times longer stimulation than the light-grown plantlets to achieve a 90° angle. The(More)
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple(More)
Mature tissue protocol for production of transgenic Citrus plants via Agrobacterium-mediated transformation uses explants derived from branches of mature, fruit-bearing trees. Through the multiple cleaning steps consisting of grafting of apical tip meristems on rootstock plants grown under sanitary conditions, "mother" plants are produced that will serve as(More)
Seedlings of Arabidopsis thaliana grown at 25°C responded to a change in growth temperature by changing their elongation rate within the next 150 min. Regardless of whether the new temperature was higher or lower than 25°C, the seedlings grew slower after the transfer at all tested temperatures. When the seedlings were grown for 2 days at 11.5°C, 17.9°C,(More)