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Transgenic tobacco plants (Nicotiana tabacum cv. SR1) expressing extracellular pancreatic ribonuclease from Bos taurus and characterized by an increased level of ribonuclease activity in leaf extracts were challenged with tobacco mosaic virus. The transgenic plants exhibited a significantly higher level of protection against the virus infection than the(More)
Protein COP1 (COnstitutive Photomorphogenesis 1) is a key regulator of plant photomorphogenesis. In darkness, COP1 affects expression of more than 20% of Arabidopsis thaliana genes; primarily, this is associated with the involvement of COP1 in proteolysis of light-controlled transcription factors; COP1 suppresses expression of photomorphogenesis genes. Some(More)
According to the scanning model, 40S ribosomal subunits initiate translation at the first (5' proximal) AUG codon they encounter. However, if the first AUG is in a suboptimal context, it may not be recognized, and translation can then initiate at downstream AUG(s). In this way, a single RNA can produce several variant products. Earlier experiments suggested(More)
The normal course of meiosis depends on regular pairing of homologous chromosomes. In intergeneric hybrids, including those of wheat, there is no chromosome pairing because there are no homologs. In F1 wheat/rye hybrids, pairing is largely prevented by the pairing homoeologous1 (Ph1) gene. In its presence, there are only rare instances of pairing; most(More)
Synthetic hexaploids are bridges for transferring new genes that determine resistance to stress factors from wild-type species to bread wheat. In the present work, the method of developing the spring bread wheat variety Pamyati Maystrenko and the results of its study are described. This variety was obtained using one of the immune lines produced earlier via(More)
Pathogenesis-related (PR) proteins participate in complex plant defense responses to pathogens. It is known that members of two PR-protein families (PR4 and PR10) exhibit ribonuclease activity in some cases. These proteins were found to be able to inhibit the growth of pathogenic fungi, and ribonuclease activity is necessary for the manifestation of this(More)
The states of 18S/5S mitochondrial (mt) repeat and some chloroplast (cp) DNA regions have been investigated in alloplasmic lines of common wheat carrying cytoplasm of the barley species Hordeum marinum subsp. gussoneanum Hudson and H. vulgare L. and in the progenies of the reciprocal hybrids between Triticum aestivum L. and Secale cereale L. The(More)
Extracellular Serratia marcescens nuclease is an extremely active enzyme which non-specifically degrades RNA and DNA. Its antiviral activity was previously shown both in animals and in plants when applied exogenously. Transgenic tobacco plants (Nicotiana tabacum L. cv. SR1) expressing S. marcescens chimeric, mutant, and intracellular mutant nuclease gene(More)