Vladimir Balabanov

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The mercury-resistance transposon Tn5053 inhibits restriction activity of the type I restriction-modification endonuclease EcoKI in Escherichia coli K12 cells. This is the first report of antirestriction activity of a non-conjugative transposon. The gene (ardD) coding for the antirestriction protein has been cloned. The ardD gene is located within the tniA(More)
Conjugative plasmids and conjugative transposons contain the genes, whose products specifically inhibit the type-I restriction-modification systems. Here, it is shown that non-conjugative transposons Tn21, Tn5053, Tn5045, Tn501, and Tn402 partially inhibit the restriction activity of the type-I restriction-modification endonuclease EcoKI (R2M2S1) into(More)
Antirestriction proteins ArdA and ArdB are specific inhibitors of type I restriction-modification enzymes. The ardA and yfeB (ardB) genes were cloned from the transmissible plasmid R64 in the pUC18 and pZE21 vectors. The R64 ArdA and ArdB proteins were shown to inhibit only restriction activity of the type I restriction-modification enzyme (EcoKI) in(More)
Luminescence intensity of recombinant Escherichia coli and Bacillus subtilis strains with cloned luxCD(AB)E genes of the natural luminescent microorganism Photobacterium leiognathi was studied under the influence of 30 individual samples of human blood serum of different component composition. A relationship was found between the level of residual(More)
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