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Signal recognition particle (SRP) is a ribonucleoprotein consisting of six distinct polypeptides and one molecule of small cytoplasmic 7SL RNA. It was previously shown to promote the co-translational translocation of secretory proteins across the endoplasmic reticulum by (a) arresting the elongation of the presecretory nascent chain at a specific point, and(More)
Signal recognition particle (SRP), a small ribonucleoprotein required for targeting secretory proteins to the ER, has three known functions: signal recognition, elongation arrest, and translocation promotion. Because SRP is inactivated by the sulfhydryl alkylating reagent N-ethylmaleimide (NEM), we have attempted to establish structure-function(More)
We have developed an assay in which incomplete preprolactin chains of varying lengths are targeted to the endoplasmic reticulum (ER) membrane in an elongation independent manner. The reaction had the same molecular requirements as nascent chain translocation across the ER membrane, namely, it was signal recognition particle (SRP) dependent, and required the(More)
  • H Flores, A Atkinson, S Kawaguchi, B A Krafft, G Milinevsky, S Nicol +24 others
  • 2012
Resale or republication not permitted without written consent of the publisher INTRODUCTION The Southern Ocean habitat of Antarctic krill Euphausia superba (hereafter 'krill') is subject to ongoing environmental changes, such as sea ice decline (Stammerjohn et al. 2008), temperature rise ABSTRACT: Antarctic krill Euphausia superba (hereafter 'krill') occur(More)
Alu-like elements comprise the most abundant family of interspersed repetitive sequences in primates and rodents, and contain many features of processed genes, suggesting that they were initially derived by reverse transcription of processed RNA transcripts. Transcripts containing Alu family members are represented in heterologous nuclear RNAs, cytoplasmic(More)
  • D C Washington, Bruce M Alberts, Wm A Wulf, Thomas R Cech, Sean R Eddy, Steven H Holtzman +66 others
  • 2003
committee's findings. Although the substance of the report is final, editorial changes may be made throughout the text prior to publication. The final report will be available through the National Academies Press later this year. The members of the committee responsible for the report were chosen for their special competences and with regard for appropriate(More)
Biochemical mutagenesis-alteration or removal of specific domains within a biological structure-followed by functional analysis, gives insight into structure-function relationships. We describe the analysis of the signal recognition particle, a ribonucleoprotein known to be required for the entry of most proteins into the secretory pathway, as an example of(More)
W elcome to PLoS Biology. We would like to introduce you to your journal, one that is run by and for the scientific community in the broadest sense: researchers, teachers, students, physicians, and the public. One could argue whether scientists need more journals, but we believe there is a global need for greater access to scientific and medical information(More)
As summarized in this minireview, two different signal recognition events, one involving SRP and the other involving proteoliposomes containing the Sec61p complex, have been identified. In cotranslational protein transport, it seems that both recognition events are required for efficient translocation of the protein into the lumen of the ER. The requirement(More)