Vivek Upadhyay

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T-cell infiltration in primary colon tumors is associated with improved patient survival. Preliminary data supports a similar association in colorectal liver metastases (CRLM), and we previously identified increased CRLM expression of the immunostimulatory cytokine LIGHT (TNFSF14) to be related to improved patient prognosis. Therefore, mechanisms to augment(More)
A comparative study with three conventional extraction techniques namely protein precipitation (PP), liquid-liquid extraction (LLE) and solid phase extraction (SPE) has been demonstrated to assess the magnitude of matrix interference by post-column analyte infusion and post extraction analyte spiking for the determination of atazanavir from human plasma.(More)
A simple, sensitive and high throughput liquid chromatography/positive-ion electrospray ionization mass spectrometry (LC-ESI-MS/MS) method has been developed for the simultaneous determination of valacyclovir and acyclovir in human plasma using fluconazole as internal standard (IS). The method involved solid phase extraction of the analytes and IS from 0.5(More)
A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid-liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasma samples with ketoconazole as internal standard (IS) were(More)
In the present paper we have formulated the renal Systolic blood flow in arteries. Keeping in view the nature of renal circulatory system in human body. The viscosity increases in the arterioles due to formation of roulex along axis by red blood cells, as we know the arteries are remote from heart and proximate to the kidney. P.N. Pandey and V. Upadhyay(More)
BACKGROUND This study evaluates the performance of three electrospray ionization source designs to monitor the interference of plasma phospholipids for reliable estimation of rivastigmine by LC-MS/MS for method ruggedness. The variation in the area response due to matrix effects was assessed by post-column infusion, post-extraction spiking and standard-line(More)
BACKGROUND The present work reports an ex vivo conversion study of prothionamide to its active metabolite prothionamide sulfoxide in human plasma during sample preparation by three conventional extraction techniques. RESULTS The chromatography was done on a Hypersil™ Gold C18 (50 × 2.1 mm, 3.0 µm) column using 0.1% acetic acid and acetonitrile (20:80,(More)
A simple, sensitive and fast throughput high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method has been developed for the determination of pyrazinamide. The method involved protein precipitation of pyrazinamide and pyrazinamide-D3 as the internal standard (ISTD) in Acetic Acid in water 1%v/v: Acetonitrile (15:85v/v) from 100µL(More)
A simple, sensitive and high throughput ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) method was developed for the determination of tacrolimus in the whole blood of Wistar rats and humans. Sample preparation involved protein precipitation of the analyte, using sirolimus as the internal standard with ZnSO4 from 50 µL of(More)
In this paper, we have presented a model of two phased blood flow in renal arterioles remote from the heart and proximate to the Kidney keeping in view the nature of renal blood circulation in human body. If blood flows arterioles to capillaries then blood pressure drop arises in human body the viscosity increases in the arterioles due to formation of(More)
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