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Recombinant DNA containing sequences of HCV NS4 protein was expressed in Escherichia coli cells. Six hybridoma clones producing monoclonal antibodies (MAB) to recombinant NS4 protein (rNS4), aa 1677-1756, were developed. Mapping with a panel of 33 peptides and reciprocal competitive EIA have shown that MAB obtained revealed five antigen determinants, not(More)
Hepatitis C virus (HCV) is of widespread occurrence in the human population (about 170 million people are infected with HCV worldwide) and is a major cause of chronic liver diseases that often lead to cirrhosis or hepatocellular carcinoma [1]. Regardless of the progress in studying the virus [2], many aspects of hepatitis C pathogenesis remain unclear.(More)
Study of prevalence of hepatitis G virus (HGV) markers in different risk groups showed the presence of HGV RNA in 3.2% blood donors, 24.2% patients with hepatitis C (HCV), and 28% patients with hemophilia. HGV antibodies were detected in 11.3% donors, 16.0% patients with HCV, 13.4% patients with hemophilia, and 8.5% HIV-infected subjects. Anti-E1 HGV were(More)
Bioinformation techniques were used to analyze envelope glycoprotein of tick-borne encephalitis virus in order to determine the potential diagnostically significant antigenic clusters. Five selected regions of the amino acid sequence of protein E were retranslated to nucleic sequences, by employing the optimum code for E. coli. The resultant DNA fragments(More)
A new enzyme immunoassay EIA-HCV-Spectr test system constructed on the base of recombinant proteins and synthetic peptides allows separate detection of antibodies to E1/E2, core, HS3, NS4, and NS5 antigens of hepatitis C virus (HCV). The system is highly specific and more sensitive than the test systems used in screening studies, which allows its use as a(More)
The study was undertaken to comparatively examine the efficiency of HIV antibody detection by enzyme immunoassay using recombinant antigens and synthetic peptides containing the most immunodominant region of the human immunodeficiency virus envelop protein gp41-cytotoxic T-lymphocytic (CTL) epitope. The application of the synthetic peptides that mimic this(More)
Solid-phase synthesis of three linear peptides overlapping the major immunodominant epitope of HDAg, which located within amino acid (aa) segment 53-108, and peptide aa 167-179 was carried out. The structure of the peptides corresponded to the sequences of isolates HDV type I. Their antigen reactivity was studied by indirect enzyme immunoassay with sera(More)
Nine peptides from immunodominant (53-68 and 65-80 aa) and hypervariable (201-213 aa) regions derived from delta antigen sequences corresponding to 3 HDV genotypes were synthesized. Type specificity of antibodies to the resultant peptides was evaluated by indirect enzyme immunoassay with sera from patients with hepatitis D and asymptomatic carriers of(More)