Vitaly N. Nikandrov

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Scavengers of different active oxygen species affect fibrin plate lysis, catalysed by various proteinases, only at relatively high concentrations (> 10(-2) M). Singlet oxygen scavengers change proteinase activity insignificantly except for strong inhibition of pepsin and papain by sodium azide, but pepsin-by histidine, and fibrinolytic urokinase activity-by(More)
The aim of this study was to determine the effect of streptokinase (SK) on the ultrastructure of cellular elements in the cerebral cortex of newborn rats in vitro. Three series of cell cultures grown on DMEM were used, including those grown on the medium enriched with 15% fetal calf serum (control 1), cultures transferred to the depleted medium containing(More)
The formation of stable equimolar complexes of streptokinase or plasminogen with muscle lactate dehydrogenase or pyruvate kinase, heart mitochondrial malate dehydrogenase and hepatic catalase at pH 7.4, 3.0 and 10.0 was first detected by differential spectroscopy methods. All complexes, except those of plasminogen with dehydrogenases, were resistant to 6 M(More)
The rabbits with CCl4-induced hepatic failure have revealed changes in hemostasis responses to streptokinase administration. The main distinction of hepatic dystrophy was the depression of plasma fibrinolytic activity accompanying the decrease in fibrinogen and antiplasmin concentrations. Streptokinase administration to rabbits with productive inflammatory(More)
Modification of tyrosine residues was detected in streptokinase molecule during iodination. A nonlinear type of the modification reaction allowed to suppose that tyrosine residues were heterogenous. As shown by gel chromatography the modified streptokinase did not develop stable complexes with human plasminogen even at low ratios of iodine/streptokinase,(More)
In the protein-deficient media plasminogen stimulated the vital functions of cells and in concentrations 10(-7)-10(-10) M it protected cells of sympathetic ganglia, neocortex and continues cell lines under damaging actions of H2O2 (0.0001 M), NH4CI (0.01 M) and cooling. Streptokinase essentially influenced the mode of damaging effect of ATP(0.001 M). Even a(More)
Human, rabbit and bovine plasminogens, having different sensitivity to streptokinase-activating action, differ, according to spectrophotometric titration, tryptophan fluorescence and circular dichroism spectroscopy, in the state of tyrosine and tryptophan residues, and in secondary and tertiary structures. Human plasminogen-streptokinase equimolar complex(More)
New analogues of the Gly-Pro-Arg and Arg-Gly-Asp fragments of fibrinogen were synthesized: Gly-Pro-Arg-Pro (I), Gly-Pro-Arg-Pro-Met-OMe (II), Gly-Pro-Arg-Pro-Phe (III), Gly-Pro-Arg-Pro-Asp (IV), Gly-Pro-Arg-Pro-Glu (V), and Arg-Asn-Trp-Asp (VI). Their effect on the activity of proteases of various types was studied with the method of lysis of fibrin plates.(More)
It was shown previously [3] that the ~nitial stages of interaction between influenza virus and the cell are accompanied by oxidation-reduction reactions catalyzed by cellular and viral enzymes. One results of this may be the intensification of peroxidation of cell membrane lipids, leading to accumulation of hydroperoxides. Inhibition of several(More)
In the article the results of the long-term researches revealing the essence of the following three phenomena are generalized. 1. Participation of active oxygen species (especially, superoxide radical) in activation of zymogens--plasminogen, trypsinogen, chymotrypsinogen, pepsinogen and in realization of the catalytic (proteolytic) function of a number of(More)