Vitali Sintchenko

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  • Marieke J. Bart, Simon R. Harris, Abdolreza Advani, Yoshichika Arakawa, Daniela Bottero, Valérie Bouchez +32 others
  • 2014
Bordetella pertussis causes pertussis, a respiratory disease that is most severe for infants. Vaccination was introduced in the 1950s, and in recent years, a resurgence of disease was observed worldwide, with significant mortality in infants. Possible causes for this include the switch from whole-cell vaccines (WCVs) to less effective acellular vaccines(More)
OBJECTIVE This study assessed the effect of a handheld computer-based decision support system (DSS) on antibiotic use and patient outcomes in a critical care unit. DESIGN A DSS containing four types of evidence (patient microbiology reports, local antibiotic guidelines, unit-specific antibiotic susceptibility data for common bacterial pathogens, and a(More)
The increasing complexity of decision-making has emerged as a risk factor in clinical medicine. The impact that decision task complexity has on the uptake and use of clinical decision support systems (DSS) is also not well understood. Antibiotic prescribing in critical care is a complex, cognitively demanding task, made under time pressure. A web-based(More)
This paper describes a model for analysing medical decision making tasks and evaluation of their suitability for automation. The overall approach focuses on the assessment of decision complexity and possible reduction of human effort by automated decision support. The approach consists of five subsequent steps: (1) selection of the domain and relevant(More)
Multilocus variable-number tandem-repeat analysis (MLVA) of 316 Bordetella pertussis isolates collected over 40 years from Australia and 3 other continents identified 66 MLVA types (MTs), including 6 predominant MTs. Typing of genes encoding acellular vaccine antigens showed changes that may be vaccine driven in 2 MTs prevalent in Australia.
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR(More)
BACKGROUND In silico candidate gene prioritisation (CGP) aids the discovery of gene functions by ranking genes according to an objective relevance score. While several CGP methods have been described for identifying human disease genes, corresponding methods for prokaryotic gene function discovery are lacking. Here we present two prokaryotic CGP methods,(More)
BACKGROUND Computational discovery is playing an ever-greater role in supporting the processes of knowledge synthesis. A significant proportion of the more than 18 million manuscripts indexed in the PubMed database describe infectious disease syndromes and various infectious agents. This study is the first attempt to integrate online repositories of(More)
Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008-2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (Prn). Multiple mechanisms of Prn inactivation were(More)
  • Simon Le Hello, Amany Bekhit, Sophie A. Granier, Himel Barua, Janine Beutlich, Magdalena Zając +18 others
  • 2013
While the spread of Salmonella enterica serotype Kentucky resistant to ciprofloxacin across Africa and the Middle-East has been described recently, the presence of this strain in humans, food, various animal species (livestock, pets, and wildlife) and in environment is suspected in other countries of different continents. Here, we report results of an(More)