Viren Sardana

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Neurophysin is a self-associating protein in which peptide-hormone binding and dimerization are thermodynamically linked. The structural basis of the linkage is unknown. We have studied the dimerization of bovine neurophysin I and two proteolytically modified derivatives by proton nuclear magnetic resonance spectroscopy in order to identify residues at the(More)
PURPOSE Cone-beam computed tomography (CBCT) is now an established component for 3D evaluation and treatment planning of patients with severe malocclusion and craniofacial deformities. Precision landmark plotting on 3D images for cephalometric analysis requires considerable effort and time, notwithstanding the experience of landmark plotting, which raises a(More)
Owing to the reduced co-relationship between conventional flat Petri dish culture (two-dimensional) and the tumour microenvironment, there has been a shift towards three-dimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was(More)
The Challenge Inequity in health care access is a major cause of increasing disease burden, catastrophic medical expenses, and inability to escape poverty, especially in the developing world [1–6]. Government programs to effect disease surveillance and treatment do exist, such as for tuberculosis, yet proper implementation is often lacking, especially in(More)
Radiation therapy (RT) is one of the modalities employed for treatment of cancer patients by irradiating the region to be treated using ionizing radiation. In radiation therapy, accurate patient positioning is an important requirement for precise dosage delivery. This paper presents a methodology for image registration employed in verification of the(More)
To evaluate the accuracy of three-dimensional cephalometric measurements obtained through an automatic landmark detection algorithm compared to those obtained through manual identification. The study demonstrates a comparison of 51 cephalometric measurements (28 linear, 16 angles and 7 ratios) on 30 CBCT (cone beam computed tomography) images. The analysis(More)
Perdeuteriated peptides were synthesized that are capable of binding to the hormone binding site of neurophysin but that differ in the position of aromatic residues. The binding of these peptides to bovine neurophysin I and its des-1-8 derivative was studied by proton nuclear magnetic resonance spectroscopy in order to identify protein residues near the(More)
The NMR behavior of the aromatic protons of bovine neurophysin-I and its complexes was interpreted with reference to the 2.8 A crystal structure of the dipeptide complex of bovine neurophysin-II and to mechanisms underlying the thermodynamic linkage between neurophysin dimerization and peptide binding. Large binding-induced shifts in the ring proton signals(More)
The proton NMR spectra and role in peptide binding of carboxyl-terminal and NH2-terminal neurophysin residues were studied by preparation of bovine neurophysin-I derivatives from which residues 90-92 had been cleaved by carboxypeptidase or residues 1-8 excised by trypsin. The carboxypeptidase-treated protein showed normal peptide-binding behavior. NMR(More)
Photoaffinity labeling of the single neurophysin tyrosine, Tyr-49, with Met-Tyr-azido-Phe amide has been reported to inhibit both neurophysin self-association and peptide binding. Accordingly, we investigated the functional consequences of modification, principally by tetranitromethane, of Tyr-49. Tetranitromethane-mediated tyrosine-tyrosine cross-linking(More)