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We have isolated a rab-related (responsive to ABA) gene, rab18 from Arabidopsis thaliana. The gene encodes a hydrophilic, glycine-rich protein (18.5 kDa), which contains the conserved serine- and lysine-rich domains characteristic of similar RAB proteins in other plant species. The rab18 mRNA accumulates in plants exposed to low temperature, water stress or(More)
Treatments as diverse as exposure to low temperature (LT), exogenous abscisic acid (ABA), or drought resulted in a 4 to 5[deg]C increase in freezing tolerance of the annual herbaceous plant Arabidopsis thaliana. To correlate the increase in freezing tolerance with the physiological changes that occur in response to these treatments, we studied the(More)
To study the role of abscisic acid (ABA) in development of freezing tolerance of Arabidopsis thaliana, we exposed wild-type plants, the ABA-insensitive mutant abi1, and the ABA-deficient mutant aba-1 to low temperature (LT), exogenous ABA, and drought. Exposure of A. thaliana to drought stress resulted in a similar increase in freezing tolerance as achieved(More)
Exogenously applied abscisic acid (ABA) induced frost hardening of Arabidopsis thaliana (L.) Heynh. The freezing tolerance of A. thaliana plantlets treated with ABA (15 mg/l) at a non-acclimating temperature (20 °C) appeared to increase even more rapidly than following a low temperature (4 °C) acclimation. Analysis of in vivo-labelled soluble proteins by(More)
Abscisic acid (ABA) has been implicated as a regulatory factor in plant cold acclimation. In the present work, the cold-acclimation properties of an ABA-deficient mutant (aba) of Arabidopsis thaliana (L.) Heynh. were analyzed. The mutant had apparently lost its capability to cold acclimate: the freezing tolerance of the mutant was not increased by low(More)
A vector was constructed for the isolation of gene fusions to thelacZ reporter gene following T-DNA integration into the genome ofArabidopsis thaliana. To facilitate the generation of taggedA. thaliana plants, we established a modified method for high-frequency transformation ofA. thaliana byAgrobacterium tumefaciens. The main modification required was to(More)
 Variation in the dehydrin gene family of ‘Himalaya’ barley (Hordeum vulgare L.) was studied using 3′ fragments of dehydrin cDNA clones (DHN1–4) as hybridisation probes. Each fragment specifically hybridised to the corresponding cDNA clone, but the 3′ fragment of DHN1 also hybridised to the very closely related DHN7 genomic clone. The same fragments were(More)
Exposure of Arabidopsis thaliana L. to an acclimation temperature (+4°C) results in a rapid increase of frost tolerance from −3°C to −7°C. This increase could be correlated to changes in soluble protein pattern. Analysis of in vitro translation products from isolated mRNA suggests that induction acts at the transcriptional level.
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