Vijay Tailor

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In vitro cell culture model systems for investigating the biochemical mechanisms involved in the neurodegenerative actions of beta-amyloid peptide (beta-AP) have been established. Using rat pheochromocytoma PC12 or human epitheloid HeLa cell lines, submicromolar concentrations of the beta-AP fragments beta 1-40, beta 1-39, and beta 25-35, but not beta 1-28,(More)
Glycosaminoglycan (GAG)-containing proteoglycans are associated with the neuritic plaques and cerebrovascular beta-amyloid deposits of Alzheimer's disease as well as with the amyloid deposits of prion and other disorders. GAGs and other sulfate-containing compounds have previously been shown to bind beta-amyloid peptide in vitro, suggesting possible effects(More)
We recently reported that several sulfate-containing glycosaminoglycans, a class of compounds associated with the beta-amyloid plaques of Alzheimer's disease, attenuate the toxic effects of beta-amyloid fragments beta 25-35 and beta 1-40. The amyloid-binding sulfonated dye Congo Red was shown to have a similar effect. Using two clonal cell lines, we now(More)
Peroxiredoxins (Prxs), proteins that detoxify peroxides, are ubiquitously present in all organisms. In the cyanobacterium Anabaena PCC7120, ORF alr4642 encodes a protein that shows homology to Prx-like family of Prxs. The role of Alr4642 in the oxidative stress management of Anabaena was evaluated in this study. Sequence analysis showed the presence of(More)
Senile plaques, a neuropathological feature of Alzheimer's disease, consist mainly of insoluble aggregates of pamyloid protein I I I . Using rat pheochromocytoma PC12 cells as an irr vitro model. i t has been shown that 8-amyloid potently inhibits the reduction of MTT 12.31. The cellular reduction of M 7 T is thought to be mediated by the mitochondrial(More)
Agents that interfere with the toxic effects of beta-amyloid protein may be therapeutically useful against Alzheimer's disease. We reported recently that several sulphated glycosaminoglycans and sulphonated dyes attenuate the toxic effects of beta-amyloid fragments beta 25-35 and beta 1-40 in two clonal cell lines. We now demonstrate that this protective(More)
The Peroxiredoxin Q (PrxQ) proteins are thiol-based peroxidases that are important for maintaining redox homeostasis in several organisms. Activity of PrxQs is mediated by two cysteines, peroxidatic (Cp) and resolving (Cr), in association with a reducing partner. A PrxQ, Alr3183, from the cyanobacterium, Anabaena PCC 7120, was characterized in this study.(More)
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