Victoria A TeBrugge

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Pericardial organs of the crayfish, Procambarus clarkii, were removed, and material from them was extracted and fractionated using two sequential reverse-phase HPLC columns. Fractions were analysed using a radioimmunoassay (RIA) specific for the C-terminal peptide sequence -Arg-Phe-NH2. The peak immunoreactive fraction from the second column appeared to(More)
Neuropeptide F (NPF)-like immunoreactivity was previously found to be abundant in the eastern subterranean termite, Reticulitermes flavipes. Purification of the NPF from a whole body extract of worker termites was accomplished in the current study by HPLC and heterologous radioimmunoassay for an NPF-related peptide, Helicoverpa zea Midgut Peptide-I. A(More)
FMRFamide-like immunoreactivity (FLI) was detected in the nervous system of the crayfish Procambarus clarkii using an antiserum that recognizes extended RFamide peptides. Immunocytochemistry revealed FLI in neuronal somata, axons and varicose processes within the central nervous system. In the periphery, plexuses of immunoreactive varicosities were present(More)
Plant sucking heteropteran bugs feed regularly on small amounts of K(+)-rich plant material, in contrast to their hematophagous relatives which imbibe large volumes of Na(+)-rich blood. It was anticipated that this would be reflected in the endocrine control of Malpighian tubule (MT) secretion. To explore this, neuroendocrine factors known to influence MT(More)
In earwigs, the male reproductive system is complex, comprising accessory glands and long dual intromittent organs for transfer of materials to the female and for removal of rival sperm. We investigated potential factors altering contractions of the male reproductive tracts in vitro. Tracts from 0-day (newly emerged) males displayed relatively little(More)
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