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Sex differences are described in both a lateralized behavior (amphetamine-elicited rotation) and in the lateralization of striatal dopamine (DA) content. Amphetamine (AMPH) elicited significantly more partial turns, total rotations and lateralized (net) rotations in female, than in male rats. The two sexes also differed in their pattern of net rotations(More)
1. There are numerous circumstantial evidence supporting the concept that steroid hormones control cellular function by means other than the nuclear receptor steroid binding mechanism. It is the intent of this report to present evidence indicating that steroids bind to specific sites in neuronal membranes. 2. Some of the criteria to define steroid membrane(More)
The push-pull perfusion technique was used in combination with a sequential bleeding schedule to estimate simultaneously the release patterns of LHRH and LH in unanesthetized ovariectomized sheep and to determine the temporal relationship between the release of these two hormones. Ovariectomized (greater than 30 days) ewes received unilateral push-pull(More)
In the present experiment we examined the temporal effects of progesterone (P) upon in vitro dopamine (DA) release from superfused corpus striatum (CS) and medial basal hypothalami (MBH) tissue fragments. In ovariectomized-estrogen-primed adult female rats, P was systemically administered at 0.5, 2, 4, 12 or 24 h prior to sacrifice for CS and at 4 or 24 h(More)
A continuous flow perifusion system was used to measure the in vitro release rate, and percent release of endogenous norepinephrine (NE) and dopamine (DA) from rat brain fragments. Catecholamines (CA) were measured simultaneously in effluent fractions and in tissue fragments following perifusion using a modified radioenzymatic assay. Employing this(More)
In the present experiment, we examined whether progesterone modifies dopamine (DA) release from superfused corpus striatal (CS) tissue fragments of ovariectomized estrogen-primed rats as result of a direct interaction with dopaminergic nerve terminals or through interneurons within the CS. In Expt. I, an in vitro pulsatile infusion of progesterone (2 ng/ml)(More)
In the present experiment we used immobilized progesterone linked to bovine serum albumin (P4-3-BSA) as a probe to examine whether the effects of a direct in vitro infusion of progesterone upon dopamine (DA) release from corpus striatal (CS) tissue fragments from ovariectomized estrogen-treated rats may be attributable to a surface membrane site of action.(More)
Previously, we have identified a membrane-mediated uptake and translocation of 1,3,5(10)-estratrien-3, 17beta-diol 6-(O-carboxymethyl)oxime:(125)I-labeled BSA (E6(125)I-BSA) in vivo in immature female rat liver from the plasma membrane (P3 fraction) to the mitochondria and/or lysosomes (P2 fraction). To further investigate this unique effect, current(More)
The present study shows that a novel, protease-sensitive factor present in a partially purified preparation from the rat adrenal gland selectively stimulates the release of dopamine from the rat striatal tissue superfused in vitro in a dose-dependent manner. Biological activity is also found to be present in much lower concentrations in the neocortex,(More)