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Four base recognition by triplex-forming oligonucleotides at physiological pH
TLDR
BAU, MeP and APP retain considerable selectivity, and single base pair changes opposite these residues cause a large reduction in affinity, in contrast, S is less selective and tolerates CG pairs as well as TA.
Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA.
TLDR
These modified triplex-forming oligonucleotides retain exquisite sequence specificity, with enhanced discrimination against YR base pairs (especially CG).
Fluorescent properties of DNA base analogue tC upon incorporation into DNA — negligible influence of neighbouring bases on fluorescence quantum yield
TLDR
The results presented here together with previous work show that tC is a very good C-analogue that induces minimal perturbation to the native structure of DNA and is thus highly interesting in a range of applications for studying e.g. structure, dynamics and kinetics in nucleic acid systems.
Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside.
TLDR
(2AE)S is the best nucleoside described so far for recognition of TA within a triple-helix target and the selectivity for TA improves with increased pH.
Recognition of CG inversions in DNA triple helices by methylated 3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues.
TLDR
Substituted 3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues have been synthesised from 5-alkynyl-uridine derivatives and found to selectively bind CG inversions with enhanced affinity compared to T.
Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides.
TLDR
UV irradiation of triplex-forming oligonucleotides (TFOs) containing internally attached psoralens produces photoadducts at TpA steps within target duplexes, thus relaxing the constraints on selection of psoralen target sequences.
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