Vernon E. Coyne

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This paper reports the first successful isolation and cell wall regeneration of Gracilaria gracilis (Stackhouse) Steentoft, Irvine et Farnham protoplasts. These results form an important foundation for the development of a successful tissue culture system for G. gracilis. Initially, an isolation protocol was optimized by investigation of the effects of the(More)
Probiotics have been widely reported to increase the growth rate of commercially important fish and shellfish by enhancing the digestion of ingested feed through the production of extracellular enzymes such as proteases and alginases. In order to investigate this further, the objective of this study was to localise the bacterial probiont Vibrio midae SY9(More)
This paper reports the first successful regeneration of whole plants from protoplasts of Gracilaria gracilis (Stackhouse) Steentoft, Irvine and Farnham. Protoplasts were isolated and purified using a previously optimized protocol. Protoplasts with regenerated cell walls divided to produce callus-like cell masses which showed the presence of uniseriate,(More)
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