Vera A. Borzova

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To search for agents affecting thermal stability of proteins, a test based on the registration of protein aggregation in the regime of heating with a constant rate was used. The initial parts of the dependences of the light scattering intensity (I) on temperature (T) were analyzed using the following empiric equation: I = K(agg)(T-T(0))(2), where K(agg) is(More)
The methodology for quantification of the anti-aggregation activity of protein and chemical chaperones has been elaborated. The applicability of this methodology was demonstrated using a test-system based on dithiothreitol-induced aggregation of bovine serum albumin at 45°C as an example. Methods for calculating the initial rate of bovine serum albumin(More)
Thermal aggregation of bovine serum albumin (BSA) has been studied using dynamic light scattering, asymmetric flow field-flow fractionation and analytical ultracentrifugation. The studies were carried out at fixed temperatures (60°C, 65°C, 70°C and 80°C) in 0.1 M phosphate buffer, pH 7.0, at BSA concentration of 1 mg/ml. Thermal denaturation of the protein(More)
R-phycoerithrin, a water soluble photosynthetic pigment of red algae, was used as a matrix and a reducer for the synthesis of Ag0 nanoparticles. The diameter of dominating Ag0 nanoparticles determined from the electron microscope images was 6.5 ± 0.5 nm. Dynamic light scattering in the regime of heating with a constant rate showed that the hydrodynamic(More)
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