Verónica Briceño

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PURPOSE Several studies have described molecular differences between microsatellite stable hereditary nonpolyposis colorectal cancer (MSS-HNPCC) and microsatellite unstable Lynch syndrome tumors (MSI-HNPCC). These differences highlight the possibility that other instability forms could explain cancer susceptibility in this group of families. The base(More)
The essential ribosomal stalk is formed in eukaryotes by a pentamer of two P1-P2 protein heterodimers and the P0 rRNA binding protein. In contrast to the highly stable prokaryotic complex, the P1 and P2 proteins in the eukaryotic stalk undergo a cyclic process of assembly and disassembly during translation that seems to modulate the ribosome activity. To(More)
The eukaryotic ribosomal proteins P1 and P2 bind to protein P0 through their N-terminal domain to form the essential ribosomal stalk. A mutational analysis points to amino acids at positions 2 and 3 as determinants for the drastic difference of Saccharomyces cerevisiae P1 and P2 half-life, and suggest different degradation mechanisms for each protein type.(More)
Plasmids Constructs carrying proteins P1 and P2 mutations First, a DNA fragment (ORF fragment) from the RPP1B and RPP2B genes containing a large stretch from the 3'UTR and the complete ORF starting at the initiator ATG from both genes, was obtained by PCR using mutagenic oligonucleotides (Table S1) at the NTD encoding region and a universal primer at the(More)
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