Venkatraman Ramakrishna

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One approach in the treatment of ovarian cancer patients involves the infusion of autologous T lymphocytes coupled with a bispecific monoclonal antibody MOv18/anti-CD3 (biMAb OC/TR), which recognizes a 38-kDa glycoprotein expressed on ovarian carcinomas and the CD3 T cell receptor. However, little is known about the in vivo biodistribution of injected(More)
This study describes a simple method for long-term establishment of human ovarian tumor lines and prediction of T-cell epitopes that could be potentially useful in the generation of tumor-specific cytotoxic T lymphocytes (CTLs). Nine ovarian tumor lines (INT.Ov) were generated from solid primary or metastatic tumors as well as from ascitic fluid. Notably(More)
The high frequency of relapse after induction chemotherapy in advanced ovarian carcinoma patients calls for new therapeutic modalities. Retargeted T cell-mediated lysis can be achieved using the bispecific antibody (BsmAb) OCTR, directed to CD3 on T cells and to the folate receptor on ovarian carcinoma cells. Twenty-eight patients with limited(More)
Multiplayer games are representative of a large class of distributed applications that suffer from redundant communication, bottlenecks and poor reactivity to changing network conditions. Many of these problems can be alleviated through simple network adaptations at the infrastructure level. In our model, game packets are directed along the edges of a tree(More)
Future environments will require devices to be automatically and safely configured to perform important tasks. Security concerns based on known vulnerabilities within the Internet make it clear that any widely deployed computing infrastructure must be designed from the start with substantially more security. However, existing security models cannot handle(More)
To provide a new tool for the immunotherapy of human ovarian carcinoma, we constructed a fusion protein between interleukin-2 (IL-2) and the single-chain Fv (scFv) of MOV19, a monoclonal antibody directed against alpha-folate receptor (alpha-FR), known to be overexpressed on human nonmucinous ovarian carcinoma. This was accomplished by fusing the coding(More)
In the preceding paper we have demonstrated an increase in presentation of both major histocompatibility complex antigens (MHC) and a tumor-associated antigen of the weakly immunogenic B16 melanoma by a straight-forward technique. The method consists in modulating the tumor cell membrane by hydrostatic pressure and simultaneous chemical crosslinking of the(More)
Delayed-type hypersensitivity (DTH) to the chemically induced EL4 and virally induced ARadLV 136 leukemia cells was determined by the radioactive ear test. Prior to the DTH test, mice were prevaccinated with cells treated either with hydrostatic pressure or with the membrane-impermeant crosslinker adenosine dialdehyde or with a combination of these. For(More)
The B16-BL6 melanoma, like most spontaneously arising tumors, is poorly immunogenic and expresses low levels of major histocompatibility complex (MHC) antigens. Treatment of cells of this tumor in vitro by hydrostatic pressure in the presence of adenosine 2',3'-dialdehyde (oxAdo), a membrane-impermeant crosslinker, caused elevated projection of MHC and a(More)
Panda is middleware designed to bring the benefits of active networks to applications not written with active networks in mind. This paper describes the architecture and implementation of Panda, and provides data on the overheads incurred and performance benefits achieved. The paper also discusses some of the key issues of automatically and transparently(More)