Venkatesh Natarajan

Learn More
For a graph (<i>V, E</i>), existing compact linear formulations for the minimum cut problem require &Theta;(|<i>V</i>||<i>E</i>|) variables and constraints and can be interpreted as a composition of |<i>V</i>|--1 polyhedra for minimum <i>s-t</i> cuts in much the same way as early approaches to finding globally minimum cuts relied on |<i>V</i>|--1 calls to a(More)
The large increase in antibody titers over the past two decades has created significant challenges for downstream processes; however, there have been no quantitative studies of the effect of feed concentration on the dynamic binding capacity in Protein A chromatography. Small scale experiments were performed using pre-packed ProSep® Ultra Plus columns over(More)
Biopharmaceutical manufacturing involves multiple process steps that can be challenging to model. Oftentimes, operating conditions are studied in bench-scale experiments and then fixed to specific values during full-scale operations. This procedure limits the opportunity to tune process variables to correct for the effects of disturbances. Generating(More)
Chromatography is an indispensable unit operation in the downstream processing of biomolecules. Scaling of chromatographic operations typically involves a significant increase in the column diameter. At this scale, the flow distribution within a packed bed could be severely affected by the distributor design in process scale columns. Different vendors offer(More)
Bubbles that rise to the surface of a cell suspension can damage cells when they pop. This phenomenon is particularly problematic in the biotechnology industry, as production scale bioreactors require continuous injection of oxygen bubbles to maintain cell growth. Previous studies have linked cell damage to high energy dissipation rates (EDR) and have(More)
Suspension mammalian cell cultures in aerated stirred tank bioreactors are widely used in the production of monoclonal antibodies. Given that production scale cell culture operations are typically performed in very large bioreactors (≥ 10,000 L), bioreactor scale-down and scale-up become crucial in the development of robust cell-culture processes. For(More)
  • 1