Venkatasamy Balasubramani

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The soil bacterium Bacillus thuringiensis is a pathogen of insects and nematodes and is very closely related to, if not the same species as, Bacillus cereus and Bacillus anthracis. The defining characteristic of B. thuringiensis that sets it apart from B. cereus and B. anthracis is the production of crystal (Cry) proteins, which are pore-forming toxins or(More)
Discovery of novel cry genes of Bacillus thuringiensis (Bt) with higher toxicity is important for the development of transgenic Bt crops resistant to target pests. Two new indigenous isolates of Bt were characterized for their colony type, crystal inclusion and toxicity with Helicoverpa armigera Hubner and Spodoptera litura Linn. Screening of cry2A genes(More)
Transgenic cotton plants expressing a novel cry2AX1 gene consisting of sequences from the cry2Aa and cry2Ac genes, driven by the CaMV35S or the EnCaMV35S promoters, were generated through Agrobacterium-mediated transformation. Among 40 independent PCR-positive events, 14 produced Cry2AX1 protein at detectable levels. Cry2AX1 levels in T0 plants ranged from(More)
In order to identify the more toxic novel cry gene, the cry1 gene was screened in six indigenous isolates of Bt by PCR with degenerate primers showed amplification in all the Bt isolates. Subsequent screening of cry1 subfamily gene(s) by gene specific primer showed amplification of cry1A gene in the five Bt isolates, three out of the six cry1 positive(More)
Bacillus thuringiensis (Bt) is popularly known as insecticidal bacterium. However, non-insecticidal Bt strains are more extensively available in natural environment than the insecticidal ones. Parasporin (PS) is a collection of genealogically heterogeneous Cry proteins synthesized in non-insecticidal isolates of Bt. An important character generally related(More)
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