Learn More
This review describes microbial transformation studies of natural drugs, its importance in correlating them with the corresponding metabolism (biotransformation) in animal system and in the structural modification of complex drug molecules, which are difficult to obtain synthetically. Emphasis is laid on the potential microorganisms, which mimic the(More)
Inhibition of biosynthetic enzymes and translation and translocation processes, elicitation, and precursor feeding were used to probe biosynthetic pathway compartmentation, substrate-product relationships, and yield limitation of the diterpenoid taxanes in cell cultures of Taxus chinensis (PRO1-95). The results suggest the following: (i) the source of(More)
Callus cultures were established from excised embryos of Nothapodytes foetida on Murashige and Skoog medium supplemented with picloram (2 mg l−1) and 3% (w/v) sucrose. The embryos developed into callus after 4 weeks of incubation at 25 ± 2 °C in dark. The cultures produced camptothecine and 9-methoxy-camptothecine as determined by TLC, UV, HPLC, electron(More)
The addition of cell extracts and cultures filtrate of Pencillium minioluteum, Botrytis cinerea, Verticillium dahliae, and Gilocladium deliqucescens on the tenth day after transferring Taxus sp. (RO1-M28) cell suspensions into an induction medium, further improved the production of Taxol Taxol is a registered trademark of Bristol Myers Squibb for(More)
Microbial biotransformations can be used to predict mammalian drug metabolism. The present investigation deals with microbial biotransformation of valdecoxib using microbial cultures. Thirty nine bacterial, fungal and yeast cultures are used to elucidate the biotransformation pathway of valdecoxib. A number of microorganisms metabolized valdecoxib to(More)
BACKGROUND Various mechanisms with a complex integrating paradigm have been implicated in diabetic complications. The present study was aimed to evaluate the aldose reductase (AR) and advanced glycation end products (AGEs) inhibitory activity of resveratrol (RSV) and its potential in the treatment of diabetic complications such as cataract and nephropathy.(More)
Diabetes is a disease, which has assumed vital public health importance because of the complications associated with it. Various mechanisms including polyol pathway along with a complex integrating paradigm have been implicated in glucose-mediated complications. Though polyol pathway was established as a major mechanism, precise pathogenesis of these(More)
Valdecoxib is a new anti-inflammatory drug that is highly selective for inhibition of the inducible form of cyclooxygenase (COX-2). In the present study, biotransformation of valdecoxib was investigated in cell cultures of five medicinal plants, viz., Catharanthus roseus, Azadirachta indica, Capsicum annuum, Ervatamia heyneana, and Nicotiana tabacum.(More)
A simple, sensitive, isocratic and reproducible reversed phase HPLC method for the determination of valdecoxib, a novel specific COX-2 inhibitor in human serum was developed using a diode array detector and celecoxib as internal standard. The system consisted of a C18 column and a detector set at 240 nm. The mobile phase was a mixture of acetonitrile:water(More)
Various mechanisms including polyol pathway along with a complex integrating paradigm with oxidative stress and advanced glycation end products (AGE) formation have been implicated in the pathogenesis of diabetic nephropathy. The present study was aimed at investigating a well known antioxidant, pterostilbene for its therapeutic role in(More)
  • 1