Vassilios Nastopoulos

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To minimize the large number of mispairs during genome duplication owing to the large amount of DNA to be synthesized, many replicative polymerases have accessory domains with complementary functions. We describe the crystal structure of replicative DNA polymerase B1 from the archaeon Sulfolobus solfataricus. Comparison between other known structures(More)
Sorcin is a 21.6 kDa calcium binding protein, expressed in a number of mammalian tissues that belongs to the small, recently identified penta-EF-hand (PEF) family. Like all members of this family, sorcin undergoes a Ca2+-dependent translocation from cytosol to membranes where it binds to target proteins. For sorcin, the targets differ in different tissues,(More)
Synthetic procedures are described that allow access to new copper(II) complexes with dipeptides containing the alpha-aminoisobutyric residue (Aib) as ligands. The solid complexes [Cu(H(-1)L(A))](n).nH(2)O (1) (L(A)H=H-Aib-Gly-OH), [Cu(H(-1)L(B))(MeOH)](n).nMeOH (2) (L(B)H=H-Aib-L-Leu-OH) and [Cu(H(-1)L(C))](n) (3) (L(C)H=H-Aib-L-Phe-OH) have been isolated(More)
Recombinant amidase is a 55.8 kDa enzyme from the thermophilic archaeon Sulfolobus solfataricus MT4 that catalyses the hydrolysis of aliphatic amides of 2-6 C atoms as well as many aromatic amides. Single crystals of purified amidase were obtained by the hanging-drop method at 294 K. Diffraction data for the native protein (2.55 A resolution) and a putative(More)
We have previously shown that a conserved glycine at position 82 of the yeast RAS2 protein is involved in the conversion of RAS proteins from the GDP- to the GTP-bound form. We have now investigated the role of glycine 82 and neighbouring amino acids of the distal switch II region in the physiological mechanism of activation of RAS. We have introduced(More)
Erabutoxin a has been crystallized in its monomeric and dimeric forms. The structures were refined at 1.50 and 1.49 A resolution, respectively, using synchrotron radiation data. The crystals belong to space group P212121, with cell dimensions a = 49.84, b = 46.62, c = 21.22 A for the monomer and a = 55.32, b = 53.54, c = 40.76 A for the dimer. Using(More)
A [Mn(III)(11)Mn(II)(6)(mu(4)-O)(8)(mu(3)-L)(4)](25+) (L = N(3)(-) or OCN(-)) octahedral unit is reported, occurring within 1D (1)(infinity) and 2D (2)(infinity) coordination polymers, as well as the corresponding 0D discrete cluster 3. It possesses a giant ground-state spin value, determined in the case of 3 to be S = 37, the second largest to be reported(More)
Two new complexes, [Zn(O(2)CPh)(2)(L)(2)].2MeOH (1.2MeOH) and [Ni(2)(O(2)CPh))(4)(L)(2)].2MeCN (2.2MeCN), have been synthesized and characterized by X-ray analysis in the course of an ongoing investigation of the M(II)/X(-)/L [M(II) = Co, Ni, Cu, Zn; X(-) = Cl(-), Br(-), I(-), NCS(-), NO(3) (-), N(3) (-), PhCO(2) (-); L = 1-methyl-4,5-diphenylimidazole](More)
The employment of the anion of methyl 2-pyridyl ketone oxime (mpko(-)) as a tridentate chelating/bridging ligand in manganese chemistry is described. The inorganic anion (Br(-), ClO(4)(-)) used in the reaction affects the identity of the product. The reaction of MnBr(2) and one equivalent of mpkoH in the presence of a base affords(More)