Vasily V. Grinev

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The aim of this study was to reveal the relationships between structure elements of flavonoids and their antileukemic activity. The human leukemia cell line K562 as a model of blast crisis of chronic myeloid leukemia and a set of 18 different flavonoids from four flavonoid subfamilies were used for researching these relationships. Relationships between(More)
BACKGROUND This study was conducted to evaluate the significance of serum level of immunoglobulins (Igs) and particularly IgG for leukemic cell persistence in peripheral blood (PB) and prognosis for childhood acute lymphoblastic leukemia (ALL). PROCEDURE Human sera were obtained from 68 children with primary B-lineage ALL at diagnosis and 46 healthy(More)
Micro RNAs are small, noncoding RNAs involved in the regulation of gene expression in eukaryotes. Being transcribed in the nucleus in the form of a long primary precursor, a primary microRNA undergoes multistep biogenesis that leads to the formation of a mature microRNA. One of the critical biogenesis steps is the recognition and processing of primary(More)
In the presented work, a new approach for the control of aml1/eto gene expression in t(8;21)(q22;q22)-positive acute myeloid leukemia cells has been developed. The technique is based on RNA-interference and lentiviral transduction methodology. Two new lentiviral vector sets for induction of constitutive anti-aml1/eto RNA-interference in acute myeloid(More)
We compared the efficiency of transduction with lentivectors based on HIV-1 and adeno-associated viruses serotype 2 and stability of transgene expression in human mesenchymal bone marrow stem cells.
This review is devoted to analyzing design rules, as well as quality controls, in the development of highly effective and specific short interfering RNA (siRNA). Four crucial steps in the development of this siRNA are discussed, i.e., selecting target RNA and designing sense and antisense strands, as well as assessing the activity and specificity of(More)
The RUNX1-RUNX1T1 fusion gene, a product of the nonhomologous balanced translocation t(8;21)(q22;q22), is a complex genetic locus. We performed extensive bioinformatic analysis of transcription initiation as well as transcription termination sites in this locus and predicted a number of different RUNX1T1 transcripts. To confirm and quantify the RUNX1T1 gene(More)
The t(8;21) translocation is the most widespread genetic defect found in human acute myeloid leukemia. This translocation results in the RUNX1-RUNX1T1 fusion gene that produces a wide variety of alternative transcripts and influences the course of the disease. The rules of combinatorics and splicing of exons in the RUNX1-RUNX1T1 transcripts are not known.(More)
The 3′ end was exactly mapped for has-mir-30a-like artificial human microRNAs that specifically recognize the mRNA of the aml1/eto fusion oncogene. The results indicated that the intracellular microRNA pool was heterogeneous in linear size relative to the 3′ end, which is necessary to consider in designing and using artificial microRNAs specific for mRNAs(More)