Vasily N. Pashkov

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This paper presents an independent comprehensive analysis of the efficiency indexes of popular open source SDN/OpenFlow controllers (NOX, POX, Beacon, Floodlight, MuL, Maestro, Ryu). The analysed indexes include performance, scalability, reliability, and security. For testing purposes we developed the new framework called hcprobe. The test bed and the(More)
A homogenous protein of 120,000 mol. wt isolated from black widow spider (Lactrodectus mactans tredecimguttatus) venom and referred to as alpha-latroinsectotoxin was highly potent (4 nM) in the induction of an increase of the frequency of miniature excitatory postsynaptic potentials in blowfly (Calliphora vicina) larvae neuromuscular preparations. In the(More)
A panel of monoclonal antibodies has been produced against alpha-latrotoxin using black widow spider venom. Five of them were characterized relative to their affinity for alpha-latrotoxin and ability to modify the main toxin effects--to increase calcium permeability of synaptosomes, to stimulate the neurotransmitter release and to form the ion channels in(More)
The unique properties of Mauthner neurons (MN), which are pairs of giant cells in the medulla oblongata of many bony fish, allow them to be used in studies of the mechanisms of neuronal plasticity at the cellular, subcellular, and molecular levels. A morphopharrnacological approach can be used for this, in which MN are exposed to known physiologically(More)
Single-channel currents produced by alpha-latrotoxin from the black widow spider venom were recorded on Xenopus oocytes injected with rat brain messenger RNA fraction of 7-8 kb. Single-channel conductance varied from 3 pS to 200 pS and sublevels of similar conductance were observed at both normal and high external concentration of Ca2+. Currents reversed at(More)
alpha-Latroinsectotoxin (LIT) from Latrodectus mactans venom increased the conductance of bilayer lipid membranes (BLM) by inducing channel like activity. The channels formed had a maximal single channel conductance of 5 pS in 10 mM CaCl2 solution. This process occurred more rapidly in symmetrical 10 mM CaCl2 solution than in equimolar KCl or NaCl. The LIT(More)
alpha-Latrotoxin, the major toxin of black widow spider venom, was suggested to bind to the specific receptor on the membrane of presynaptic cells and to activate a nonselective cation channel. The aim of this investigation was to express the receptor to alpha-latrotoxin in the membrane of Xenopus laevis oocytes. Responses to alpha-latrotoxin were studied(More)
We have studied [14C]GABA release from synaptosomes induced by native and monoclonal antibodies-modified alpha-latrotoxin (LTX). Modification of LTX eliminates the toxin's ability to increase [Ca2+]i influx into synaptosomes. It has been shown that native LTX does not change 22Na influx into rat brain synaptosomes. Both toxin forms studied, native and(More)
A review is presented of the possibilities of using Xenopus oocytes for molecular-biological studies. Combination of biochemical, electrophysiological, and molecular-genetic methods applied to a single oocyte provides exceptional conditions for getting information about the biogenesis, structure function relationships, and modulation of plasma membrane(More)
In this paper we present DYANA, an HLA-based hardware-in-the-loop simulation tool. This tool is used for distributed Real-Time Embedded Systems (RTES) simulation. RTES models are described by Unified Modeling Language (UML) statechart diagrams. The statechart diagram is transformed into HLA-based Simulation Model (HSM). After translation into HSM we use(More)