Varsha Raghavan

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Bacterial interaction with eukaryotic hosts is often mediated by classical two-component systems, where a sensor kinase controls the phosphorylated state of a cognate response regulator directly, as well as by atypical two-component systems. In the gut symbiont Bacteroides thetaiotaomicron, the sensor kinase and response regulator domains are fused into a(More)
UNLABELLED The mammalian intestine provides nutrients to hundreds of bacterial species. Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence. Here we reveal that related Bacteroides species that can utilize the mammalian glycan(More)
Cells respond to nutrient availability by expressing nutrient catabolic genes. We report that the regulator controlling utilization of chondroitin sulphate (CS) in the mammalian gut symbiont Bacteroides thetaiotaomicron is activated by an intermediate in CS breakdown rather than CS itself. We determine that the rate-determining enzyme in CS breakdown is(More)
Cellular processes require specific interactions between cognate protein partners and concomitant discrimination against noncognate partners. Signal transduction by classical two-component regulatory systems typically entails an intermolecular phosphoryl transfer between a sensor kinase (SK) and a cognate response regulator (RR). Interactions between(More)
Epoch-Era Analysis (EEA) is a quantitative analysis approach that allows decision-makers to evaluate performance of design alternatives over a set of possible futures. To address the computational and cognitive burden arising from a potentially unlimited number of futures, the insertion of human-in-the-loop interaction into certain modules of EEA, or(More)
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