Vania Ramírez-León

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Postembedding immunogold labeling was used to determine the relationship between AMPA and NMDA receptor density and size of Schaffer collateral-commissural (SCC) synapses of the adult rat. All SCC synapses expressed NMDA receptors. AMPA and NMDA receptors were colocalized in at least 75% of SCC synapses; the ratio of AMPA to NMDA receptors was a linear(More)
Motor disturbances and wasting of skeletal muscles (sarcopenia) causes significant impairment of daily life activities and is a major underlying cause for hospitalization in senescence. Herein we review data and present new findings on aging-specific changes in motoneurons, skeletal muscle and the interplay between motoneurons and target muscle fibers.(More)
The motoneurons (MNs) in the ventrolateral nucleus (VLN) of the upper sacral spinal cord segments in the cat supply the external sphincters and the ischiocavernosii muscles. The dendrites of the MNs in the VLN are arranged into rostro-caudally oriented bundles (ventrolateral dendritic bundle, VLB). In this study we describe the distribution and synaptic(More)
Indirect single- and double-staining immunofluorescence techniques were used to study the serotoninergic, peptidergic and GABAergic innervation of the ventrolateral (Onuf's nucleus) and dorsolateral (innervating intrinsic foot sole muscles) nuclei, located in the S1/S2 segments of the cat spinal cord. The relative density of 5-hydroxytryptamine-,(More)
An increasing body of evidence indicates that aging-related impairments of nervous functions are caused by damage to neuron integrity rather than by loss of neurons. By using electron microscopy, we have examined axosomatic boutons on spinal cord motoneurons derived from aged and young adult Sprague-Dawley rats. The main finding was that about half of the(More)
The distribution and synaptic arrangement of thyrotropin-releasing hormone-, substance P- and enkephalin-immunoreactive axonal boutons have been studied in the ventrolateral nucleus (Onuf's nucleus) of the upper sacral spinal cord segments in the cat. For this purpose, the peroxidase-antiperoxidase immunohistochemical technique was used. Immunoreactive(More)
Glutamate has been implicated in signal transmission between sensory hair cells and afferent fibers in the inner ear. However, the mechanisms responsible for glutamate replenishment in these cells are not known. Here we provide evidence that phosphate activated glutaminase, which is thought to be the predominant glutamate-synthesizing enzyme in the brain,(More)
OBJECTIVE The severity of postpartum psychosis calls for further research on the association between obstetric variables and this psychiatric disorder. METHOD A total of 1,133368 Swedish first-time mothers were included during a 29-year period yielding 1413 hospitalized cases of postpartum psychosis. Several obstetric variables were analysed separately(More)
articles AMPA and NMDA receptors are the major classes of ionotropic receptors in central glutamatergic synapses 1–3. There is now strong evidence from high-resolution immunogold analyses that both receptors are concentrated opposite the presynaptic active zone in a membrane domain that coincides precisely with the postsynaptic density 3–9. However , it is(More)
The spinal cord motor nuclei have been the focus of a number of investigations exploring neurodegenerative mechanisms, e.g. excitotoxicity mediated by glutamate and oxidative stress. Here, high-resolution quantitative post-embedding immunocytochemistry with antibodies to oxidized and reduced glutathione (GSH), an ubiquitously expressed scavenger of free(More)
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