Valery Terletskiy

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Pseudomonas aeruginosa is one of the leading nosocomial pathogens in intensive care units (ICUs). The source of this microorganism can be either endogenous or exogenous. The proportion of cases as a result of transmission is still debated, and its elucidation is important for implementing appropriate control measures. To understand the relative importance(More)
This study describes the application and evaluation of a recently developed fast bacterial typing technique (double digest selective label - DDSL) for hospital isolates of Pseudomonas aeruginosa. The protocol was based on a simultaneous double digestion/labelling reaction which was performed in a single reaction tube. After agarose gel separation(More)
SUMMARY A database for simulation of double digest selective label (DDSL) typing technique has been created and validated against a sequenced strain (Salmonella enterica serovar Typhimurium strain LT2). In silico bands were in agreement with experimental, and the technique was able to discriminate among strains belonging to the same species. When compared(More)
Fast microorganism genotyping technique has been developed with the use of the double distribution and selective labeling method (DDSL) for identification of disease-associated serotypes of Salmonella and Proteus strains. Isolates have been obtained from poultry tissues and dung from different places in various years. The results of strain genotyping(More)
The search for single nucleotide polymorphisms (SNPs) in the myostatin gene is a promising direction of research as this gene is involved in the development of important biological and productive traits in chickens. Using polymerase chain reaction (PCR-RFLP), allele and genotype frequencies were analyzed in the Cornish chicken breed, line G5, cross Smena-8.(More)
Polymorphic variants in the myostatin, prolactin, and D2 dopamine receptor genes were analyzed in Pushkin breed chickens (n = 231). The rs313744840 single nucleotide polymorphism was studied in the myostatin gene by means of the PCR–RFLP method. The cocks with different genotypes did not differ from each other by the live weight. Chickens with AA genotype(More)
Tracing transmission routes and identifying pathogen sources are important issues in preventive measures aimed at controlling human and animal infectious diseases. A fast and accurate method for bacterial strain identification (genotyping) allows a scientifically sound planning of preventive schemes. Despite the existence of numerous bacteri a genotyping(More)
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