Valeriy Sibirtsev

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Spectral data and nucleic acid complex formation properties for more than 30 both newly synthesized and widely used fluorescent nucleotide-specific compounds of various classes have been analyzed. These include phenylbenzene, bisbenzimidazole, psoralen, angelicin, tetrahydrocarbazole, oxophenoxazine, and others. The main rules of a generalized model(More)
Absorption and fluorescence spectra of a series of bisbenzimidazole dyes were studied in the presence of various concentrations of DNA and in diverse media. Variations in spectral properties of the compounds under study were found to depend on the measurement conditions. The bases for selecting the optimal composition of the media for DNA detection were(More)
A method for the analysis of microbial “growth curves” by real-time recording of changes in the impedance electrical conductance of the medium from multiple samples in parallel is described; these changes arise from metabolic processes taking place in the test microorganisms in the samples. Results obtained from practical application of the impedance(More)
Spectral properties of a series of externally binding substrate-specific bis-benzimidazole dyes that were either bound or not bound to DNA were studied. Feasible mechanisms of variation in fluorescent properties of the dyes under study were considered depending on their chemical structure. Contribution of the rotational diffusion and the donor-acceptor(More)
Mathematical and statistical procedures were used to identify individual response in rats to benzo(a)pyrene (BP) treatment versus dose and regimen (single or repeated administration). Primary experimental data were suitably processed to exclude extreme values. A significant correlation was established between life-span and urine level of(More)
Various models of complex formation of low-molecular ligands with DNA are considered. Using the Scatchard model, parameters of binding of fluorescent monophenylindole, monophenylbenzimidazole, and bisbenzimidazole dyes with calf thymus DNA were evaluated. By means of graphic (nonparametric) and correlation analysis, various spectral and complexation(More)
The experimental data on the effects of a widespread carcinogen, benzo[a]pyrene (BP), on individual reactions of rats were treated using mathematical—statistical methods. The individual reactions were analyzed in dependence of doses and modes of administration (single or chronic). The analysis revealed a statistically significant correlation between life(More)
The investigation goal of the present study is the possible utilization of early postradiation peripheral blood DNA content changes during radiotherapy (RT) of cancer patients for prognostic purposes related to the subsequent ascertaiment of leukopenia values. Twenty-three primary patients with II-III stage Hodgkin's disease were treated at the linear(More)