Valerie Farnsworth

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C3a purified to chemical homogeneity from human serum binds preferentially to human eosinophils greater than neutrophils. Little or no binding is found with human platelets. Maximum binding to eosinophils at 37 degrees C occurs within 15 min. Dilution of 125I-C3a by either cold C3a or washing away unbound 125I-C3a and reincubating at 37 degrees C reveals a(More)
An automated subpicomole sequencing method is described. It is based on a chemistry that generates easily detectable amino acid derivatives from the postcleavage products of the classical Edman degradation. These products, which include the amino acid anilinothiazolinone (ATZ), phenylthiocarbamyl (PTC), and phenylthiohydantoin (PTH), are converted to a(More)
The N-terminal 20 residues of 13 heavy immunoglobulin chains from myeloma protein of the BALB/c mouse are compared with the same residues of 15 other heavy chains described in the literature. Sixteen of 28 sequences are different from one another. These proteins fall into four major sets, with 18 of the proteins in the largest set being further divisible(More)
The amino acid sequence of the constant (CK) region from the kappa immunoglobulin chains of a b9 rabbit is compared with the CK sequences, taken from the literature, of a b4 rabbit. These CK regions differ by 33% of their amino acid sequences and by three sequence insertions or deletions (sequence gaps). These extensive differences together with other(More)
A method is described for the generation of phenylthiocarbamyl (PTC) amino acids from phenylthiohydrantoin (PTH) or anilinothiazolinone (ATZ) amino acids. An aqueous base containing a reducing agent promotes the opening of the PTH or ATZ ring to form a stable PTC amino acid. The new reaction is essentially quantitative for all of the amino acids and may be(More)
Baseline noise resulting from the by-products of the automated Edman degradation of proteins is reduced using nonstandard coupling chemicals and dual-wavelength diode array detection of phenylthiohydantoin (PTH) amino acids. Changes to the chemistry include lowering the phenylisothiocyanate (PITC) concentration and using a novel coupling base(More)
Proteins can be identified by amino acid analysis and database matching, but it is often desirable to increase the confidence in identity through the use of other techniques. Here we describe a rapid protein identification method that uses Edman degradation to create a 3 or 4 amino acid N-terminal "sequence tag," following which proteins are subjected to(More)
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