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BACKGROUND & AIMS The preoperative diagnosis of cholangiocarcinoma is associated with a low sensitivity. To overcome this limitation, a new imaging modality was evaluated to detect neoplasia in vivo in the biliary tract. METHODS Fourteen patients with biliary strictures were examined. Mucosal imaging was performed with a miniaturized confocal laser(More)
BACKGROUND AND STUDY AIMS The accurate diagnosis of indeterminate pancreaticobiliary strictures presents a clinical dilemma. Probe-based confocal laser endomicroscopy (pCLE) offers real-time in vivo microscopic tissue examination that may increase sensitivity for the detection of malignancy. the objective of this study was to develop and validate a standard(More)
OBJECTIVE The biggest challenge in endoscopic surveillance of Barrett's oesophagus is better detection of neoplasia in mucosa of normal macroscopic appearance. We evaluated in vivo miniprobe confocal laser microscopy (CLM) for the detection of invisible Barrett's neoplasia. DESIGN Prospective two-centre trial in two phases: phase I to establish criteria(More)
BACKGROUND & AIMS Confocal fluorescence microscopy (CFM) has been mentioned to be a promising tool for in vivo histology. Recently, a portable confocal miniprobe has been developed. Our aim was to evaluate the potential benefit of CFM for detection of gastrointestinal neoplasia. METHODS A total of 47 patients with known or suspected neoplasia in the upper(More)
BACKGROUND AND STUDY AIMS Surveillance of Barrett's esophagus includes endoscopic inspection with biopsy of suspicious lesions followed by four-quadrant biopsy of the remaining mucosa. We assessed the ability of probe-based confocal laser endomicroscopy (pCLE) to replace biopsy in the endoscopic evaluation of patients with Barrett's esophagus in a(More)
BACKGROUND Confocal laser scanning microscopy (CLSM) is reported to be a promising tool for in vivo histopathology during an endoscopy. The most commonly used fluorophore is fluorescein sodium given intravenously. However, so far, there are no objective data on contrast dynamics and image quality over time after injection of the substance. OBJECTIVE We(More)
BACKGROUND Probe-based confocal laser scanning endomicroscopy enables in vivo real time histopathology of the mucosa layer. Recently, a prototype of a new confocal miniprobe has been developed that is small enough to be introduced through a 22-gauge puncture needle. OBJECTIVE The aim of this study was to evaluate the feasibility of such needle-based(More)
BACKGROUND A new portable confocal laser microscopy system has recently been developed. OBJECTIVE Our purpose was to evaluate the feasibility of performing real-time microscopic imaging with a prototype of a new high-resolution miniprobe in conjunction with a video mosaicing algorithm. DESIGN Feasibility study. SETTING Tertiary referral center at a(More)
BACKGROUND AND STUDY AIMS Angiogenesis has been reported to be an essential step in the progression of cancers arising from Barrett's esophagus. Confocal laser scanning microscopy (CLM) has the potential to perform in vivo microscopy to detect angiogenesis and determine microvessel density (MVD). We aimed therefore to use this new promising imaging tool for(More)
Loss of p53 is considered to allow progression of colorectal tumors from the adenoma to the carcinoma stage. Using mice with an intestinal epithelial cell (IEC)-specific p53 deletion, we demonstrate that loss of p53 alone is insufficient to initiate intestinal tumorigenesis but markedly enhances carcinogen-induced tumor incidence and leads to invasive(More)