Valdis A. Dzelzkalns

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The promoter of the S Locus Glycoprotein (SLG) gene of Brassica is a tightly regulated promoter that is active specifically in reproductive organs. In transgenic tobacco, this promoter is active exclusively in cells of the pistil and in pollen. We transformed tobacco with truncated versions of the SLG13 promoter fused to the beta-glucuronidase reporter(More)
Photosynthesis-defective mutants of the transformable cyanobacterium Synechocystis 6803 have been isolated following nitrosoguanidine mutagenesis. The photosystem II- phenotype of one of these mutants is shown by DNA sequencing to be attributable to a short deletion in psbC, the gene encoding the 44-kd, chlorophyll-binding protein of photosystem II.(More)
The putative promoter region of the chloroplast encoded ps2B gene (the gene encoding the 32kD herbicide binding B protein of photosystem II (1-4)) has been fused to a chloramphenicol acetyl transferase (CAT) gene that lacks its bacterial promoter and found to accurately initiate transcription from this promoter when introduced into the cyanobacterium,(More)
We describe the cloning and characterization of PCP, a novel calcium-binding protein that is expressed predominantly in the pistils and anthers of Brassica flowers late in flower development. A PCP cDNA – isolated from a subtracted cDNA library enriched in transcripts present in the pistil late in flower development – potentially encodes a 175 amino acid(More)
delta-Aminolevulinic acid (ALA) synthase [succinyl-CoA:glycine C-succinyltransferase (decarboxylating), EC 2.3.1.37] activity was detected in cell extracts of the unicellular green flagellate alga Euglena gracilis. The enzyme was identified by substrate and cofactor requirements, and activity was proportional to number of cells extracted and duration of(More)
Chloroplasts and cyanobacteria contain genes encoding polypeptides homologous to some subunits of the mitochondrial respiratory NADH-ubiquinol oxidoreductase complex (NADH dehydrogenase). Nothing is known of the role of the NADH dehydrogenase complex in photosynthesis, respiration, or other functions in chloroplasts, and little is known about the specific(More)
delta-Aminolevulinic acid (ALA), a key precursor of the tetrapyrroles heme and chlorophyll, is capable of being synthesized by two different routes in cells of the unicellular green alga Euglena gracilis: from the intact carbon skeleton of glutamate, and via the condensation of glycine and succinyl CoA, mediated by the enzyme ALA synthase. The regulatory(More)
Self-incompatibility, a mechanism that prevents self-fertilization in plants, is based on the ability of the pistil to discern the presence of self-pollen and on the female tissue's capacity to inhibit the growth or germination of self-related, but not of genetically unrelated, pollen. As a self-recognition system, self-incompatibility responds to specific(More)
The polypeptide composition and spectral properties of three photosystem II (PSII) deficient mutants of the cyanobacterium Synechocystis 6803 have been determined. The levels of the 43 and 47 kilodalton chlorophyll-binding proteins and the reaction center component D2 are affected differently in each mutant; the 33 kD polypeptide of the oxygen-evolving(More)
Irradiation of the photoheterotrophic cyanobacterium Synechocystis sp. PCC 6803 with low levels of UV light allows for stable, integrative transformation of these cells by heterologous DNA. In this system, transformation does not rely on an autonomously replicating plasmid and is independent of homologous recombination. Cells treated with UV light in the(More)