Learn More
AFLP™ is one of the most frequently used techniques for identification of molecular markers. We have modified the procedures for genomic DNA extraction, AFLP product generation and silver staining in order to speed up analyses and screen large numbers of plant samples. Using this protocol, we were able to achieve an 82% reduction of costs without(More)
To quantify genetic diversity among modern and earlier maize cultivars, 133 varieties, representative of the maize grown in France during the last five decades, were fingerprinted using 51 SSR. The varieties were grouped into four periods. For each period, allelic richness, genetic diversity and genetic differentiation among periods were computed. A total(More)
In most production areas, Alternaria leaf blight (ALB) is recognized as the most common and destructive foliage disease in carrot. To assess the genetic architecture of carrot ALB resistance, two parental coupling maps were developed with similar number of dominant markers (around 70), sizes (around 650 cM), densities (around 9.5 cM), and marker(More)
Small, finite populations are particularly vulnerable to diversity loss during regeneration. The regeneration of a highly outbreeding open-pollinated variety relies on estimated effective population size, via the measurement of temporal change in allele frequencies. Using appropriate estimators for dominant gene markers, effective sizes were calculated for(More)
When genetic diversity among organisms was measured with molecular markers, the question of genome coverage was currently stressed out. In order to check if well-distributed, mapped AFLP markers were more efficient in assessing varietal identification of carrot accessions than randomly chosen markers, nine closely related genotypes were analysed. A software(More)
The aim of this study, led by the GEVES (Research and Control Group for Varieties and Seeds), was to suggest indicators to assess the diversity available to farmers since the French Official Catalogue for Plant Varieties and Species was initiated. The largest datasets of 1990 inbred maize lines and 578 pea lines from the last 50 years were analysed using(More)
Molecular markers have proved their efficiency for the identification of duplicate accessions in genetic resources collections. Partners of the GENRES Carrot project decided to evaluate the use of molecular markers for the identification of carrot accession duplicates. As a model analysis, 21 presumed duplicate accessions of ‘Jaune du Doubs’ were selected.(More)
The interaction between Alternaria dauci and two carrot cultivars differing in their resistance to leaf blight was investigated by microscopy. The fungal development between 1 and 15 days post-inoculation was quite similar in the susceptible cv. Presto and the partially resistant cv. Texto: After conidial germination, leaf adhesion of the pathogen was(More)
Horizontal and polygenic resistance is race-nonspecific and, therefore, more durable, unlike vertical resistance, which is race-specific and unstable. However, this division is perhaps not so obvious since some cultivar × isolate interactions have already been observed for plant species with partial resistance. Carrot is known to be partially resistant to(More)
Combining biparental and multiparental connected population analyses was useful for the identification of 11 QTLs in two new genetic backgrounds of carrot resistance to Alternaria dauci and for breeding recommendations. Leaf blight due to the fungus Alternaria dauci is the major carrot foliar disease worldwide. Some resistance QTLs have been previously(More)
  • 1