Patterns of histone binding to DNA of transcriptionally active D. melanogaster hsp70 genes within the nuclei have been analyzed by two methods of histone-DNA chemical cross-linking. When cross-linking is restricted to the central, "globular" regions of histones, it drops most for H1, to an intermediate extent for H2A and H2B, and least for H3 and H4 in… (More)
Brain deterioration resulting from "protein folding" diseases, such as the Alzheimer's disease (AD), is one of the leading causes of morbidity and mortality in the aging human population. Heat shock proteins (Hsps) constitute the major cellular quality control system for proteins that mitigates the pathological burden of neurotoxic protein fibrils and… (More)
The presence of histones in hsp 70 genes was studied by "protein-image" hybridization technique after crosslinking histones to DNA. With increasing transcription of the genes, the coding region was demonstrated to be depleted first of H1 and then of all histones. This probably accounts for unraveling the 25 nm silent chromatin fiber to moderately and… (More)
Methyl methanesulfonate (MMS) is an alkylating agent commonly used in models of genotoxic stress. It methylates bases in DNA, but also leads to oxidative stress. The transcription factor Rpn4 protects yeast cells from toxic effect of MMS. Although Rpn4 is a major regulator of ubiquitin-proteasome system (UPS), a number of data points to its participation in… (More)
The review describes the changes observed in long noncoding RNA (lncRNA) content and function at various stages of carcinogenesis, as well as the prospects of lncRNA application in cancer prognosis.
A new immuno-PCR format based on the detection of CDH17 membrane protein in serum exosomes is proposed. Using the technique described, it was possible to distinguish between serum specimens obtained from healthy donors and colon cancer patients. The results suggest that the new technique may enable the serological diagnosis of colon cancer in high-risk… (More)
The review summarizes our current knowledge on the role of tumor-derived exosomes and microvesicles in the progression, metastasis, and angiogenesis of tumors, as well as in the suppression of adaptive and innate immunity.
Processing of mycobacterial antigens by immunoproteasome is necessary to control infection and to protect the organism from the development of active tuberculosis. We have investigated the activation of transcription of immunoproteasome subunit genes in peritoneal monocytes of C57Bl/6 mice infected with a vaccine M. bovis strain BCG and a virulent M.… (More)
This review describes the most popular methods of searching for serological markers of tumors that are used in a clinical setting, as well as a comparison of their efficiency.
The production of major human heat shock protein Hsp70 (HSPA1A) in a eukaryotic expression system is needed for testing and possible medical applications. In this study, transgenic mice were produced containing wild-type human Hsp70 allele in the vector providing expression in the milk. The results indicated that human Hsp70 was readily expressed in the… (More)