Vadim L. Karpov

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Patterns of histone binding to DNA of transcriptionally active D. melanogaster hsp70 genes within the nuclei have been analyzed by two methods of histone-DNA chemical cross-linking. When cross-linking is restricted to the central, "globular" regions of histones, it drops most for H1, to an intermediate extent for H2A and H2B, and least for H3 and H4 in(More)
Brain deterioration resulting from "protein folding" diseases, such as the Alzheimer's disease (AD), is one of the leading causes of morbidity and mortality in the aging human population. Heat shock proteins (Hsps) constitute the major cellular quality control system for proteins that mitigates the pathological burden of neurotoxic protein fibrils and(More)
The sequential arrangement of histones along DNA in nucleosome core particles was determined between 0.5 and 600 mM salt and from 0 to 8 M urea. These concentrations of salt and urea up to 6 M had no significant effect on the linear order of histones along DNA but 8 M urea caused the rearrangement of histones. Conformational changes in cores have been(More)
The presence of histones in hsp 70 genes was studied by "protein-image" hybridization technique after crosslinking histones to DNA. With increasing transcription of the genes, the coding region was demonstrated to be depleted first of H1 and then of all histones. This probably accounts for unraveling the 25 nm silent chromatin fiber to moderately and(More)
Methyl methanesulfonate (MMS) is an alkylating agent commonly used in models of genotoxic stress. It methylates bases in DNA, but also leads to oxidative stress. The transcription factor Rpn4 protects yeast cells from toxic effect of MMS. Although Rpn4 is a major regulator of ubiquitin-proteasome system (UPS), a number of data points to its participation in(More)
We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be(More)
A new immuno-PCR format based on the detection of CDH17 membrane protein in serum exosomes is proposed. Using the technique described, it was possible to distinguish between serum specimens obtained from healthy donors and colon cancer patients. The results suggest that the new technique may enable the serological diagnosis of colon cancer in high-risk(More)