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Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
A collection of Tn5-derived minitransposons has been constructed that simplifies substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the introduction ofExpand
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Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.
A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition featuresExpand
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Opening the iron box: transcriptional metalloregulation by the Fur protein.
It is generally accepted that iron is the most important micronutrient used by bacteria. With members of the family Lactobacillae being the only exceptions so far (3), this metal is essential forExpand
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Transcriptional Tradeoff between Metabolic and Stress-response Programs in Pseudomonas putida KT2440 Cells Exposed to Toluene*
When Pseudomonas putida KT2440 cells encounter toluene in the growth medium, they perceive it simultaneously as a potential nutrient to be metabolized, as a membrane-damaging toxic drug to beExpand
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Export of autotransported proteins proceeds through an oligomeric ring shaped by C‐terminal domains
An investigation was made into the oligomerization, the ability to form pores and the secretion‐related properties of the 45 kDa C‐terminal domain of the IgA protease (C‐IgAP) from NeisseriaExpand
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Binding of the fur (ferric uptake regulator) repressor of Escherichia coli to arrays of the GATAAT sequence.
The mode of DNA binding of the Fur (ferric uptake regulator) repressor which controls transcription of iron-responsive genes in Escherichia coli, has been re-examined. Using as a reference the knownExpand
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Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440.
The genome of the soil bacterium Pseudomonas putida strain KT2440 has been erased of various determinants of resistance to antibiotics encoded in its extant chromosome. To this end, we employed aExpand
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Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways*
Background: Glucose metabolism in many bacteria is based on the standard linear Embden-Meyerhof-Parnas pathway. Results: Pseudomonas putida operates a cycle merging components of the Entner-DoudoroffExpand
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Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor.
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequenceExpand
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