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Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria
A collection of Tn5-derived minitransposons has been constructed that simplifies substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the introduction of…
Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
The cloning system described here will be particularly useful for the construction of hybrid bacteria that stably maintain inserted genes, perhaps in competitive situations, and that do not carry antibiotic resistance markers characteristic of most available cloning vectors.
Opening the Iron Box: Transcriptional Metalloregulation by the Fur Protein
It is generally accepted that iron is the most important micronutrient used by bacteria, but this element is not easily available to microorganisms in aerobic environments, so iron uptake has to be exquisitely regulated to maintain the intracellular concentration of the metal between desirable limits.
Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence…
Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440.
The directed edition of the P. putida chromosome shown here not only enhances the amenability of this bacterium to deep genomic engineering, but also validates the corresponding approach for similar handlings of a large variety of Gram-negative microorganisms.
Binding of the fur (ferric uptake regulator) repressor of Escherichia coli to arrays of the GATAAT sequence.
DNase I and hydroxyl radical footprinting, as well as missing-T assays, consistently revealed that functional Fur sites are composed of a minimum of three repeats of the hexameric motif GATAAT rather than by a palindromic 19 bp target sequence.
Export of autotransported proteins proceeds through an oligomeric ring shaped by C‐terminal domains
It is reported that purified C‐IgAP forms an oligomeric complex of ∼500 kDa with a ring‐like structure containing a central cavity of ∼2 nm diameter that is the conduit for the export of the N‐doms.
Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes.
Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.
Not every function of DNA bends is understood, but their presence has been detected in a wide variety of bacterial promoters subjected to positive or negative control.
Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways*
- P. Nikel, M. Chavarría, Tobias Fuhrer, U. Sauer, V. de Lorenzo
- BiologyJournal of Biological Chemistry
- 8 September 2015
Determination of the NADPH balance revealed that the default metabolic state of P. putida KT2440 is characterized by a slight catabolic overproduction of reducing power, which may contribute to the physiological heftiness of this bacterium in its natural habitats.