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Substrate Recognition and Activity Regulation of the Escherichia coli mRNA Endonuclease MazF*♦
Escherichia coli MazF (EcMazF) is the archetype of a large family of ribonucleases involved in bacterial stress response. The crystal structure of EcMazF in complex with a 7-nucleotide substrateExpand
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Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics
The Staphylococcus aureus genome contains three toxin–antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-typeExpand
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Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
Toxin-antitoxin (TA) modules are pairs of genes essential for bacterial regulation upon environmental stresses. The mazEF module encodes the MazF toxin and its cognate MazE antitoxin. The highlyExpand
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An efficient method for the purification of proteins from four distinct toxin-antitoxin modules.
Toxin-antitoxin (TA) modules are stress response elements that are ubiquitous in the genomes of bacteria and archaea. Production and subsequent purification of individual TA proteins is anything butExpand
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Structural Basis of the Subcellular Topology Landscape of Escherichia coli
Cellular proteomes are distributed in multiple compartments: on DNA, ribosomes, on and inside membranes, or they become secreted. Structural properties that allow polypeptides to occupy subcellularExpand
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1H, 13C, and 15N backbone and side-chain chemical shift assignment of the staphylococcal MazF mRNA interferase
MazF proteins are ribonucleases that cleave mRNA with high sequence-specificity as part of bacterial stress response and that are neutralized by the action of the corresponding antitoxin MazE.Expand
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Structural insights into the binding of natural pyrimidine-based inhibitors of class II aminoacyl-tRNA synthetases.
The pyrimidine-containing Trojan horse antibiotics albomycin and a recently discovered cytidine-containing microcin C analog target the class II seryl- and aspartyl-tRNA synthetases (serRS and aspR...
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Crystallization of the Staphylococcus aureus MazF mRNA interferase.
mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute aExpand
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The Preprotein Binding Domain of SecA Displays Intrinsic Rotational Dynamics.
SecA converts ATP energy to protein translocation work. Together with the membrane-embedded SecY channel it forms the bacterial protein translocase. How secretory proteins bind to SecA and driveExpand
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Structural insights into the binding of natural pyrimidine-based inhibitors of class II aminoacyl-tRNA synthetases.
The pyrimidine-containing Trojan horse antibiotics albomycin and a recently discovered cytidine-containing microcin C analog target the class II seryl- and aspartyl-tRNA synthetases (serRS andExpand
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