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Biospecific irreversible fishing coupled with atomic force microscopy for detection of extremely low‐abundant proteins
In the absence of an analog of PCR for proteins, the concentration detection limit (DL) becomes a real challenge. The problem may be solved by means of a combination of biospecific irreversibleExpand
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Isatin‐binding proteins of rat and mouse brain: Proteomic identification and optical biosensor validation
Isatin (indole‐2,3‐dione) is an endogenous indole that has a distinct and discontinuous distribution in the brain and in other mammalian tissues and body fluids. Its output is increased underExpand
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AFM fishing nanotechnology is the way to reverse the Avogadro number in proteomics
Future development of proteomics may be hindered by limitations in the concentration sensitivity of widespread technological approaches. The concentration sensitivity limit (CSL) of currently usedExpand
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Structure-function relationships of rat liver CYP3A9 to its human liver orthologs: site-directed active site mutagenesis to a progesterone dihydroxylase.
CYP3A9 is an estrogen-inducible ortholog of human liver CYP3A4 with 76.5% sequence identity to CYP3A4. Unlike CYP3A4, it is a very poor testosterone 6beta- and 2beta-hydroxylase, but a relativelyExpand
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Proteomic profiles of induced hepatotoxicity at the subcellular level
In the present study proteomes of liver samples were analyzed after administration of phenobarbital (PB) or 3‐methylcholantrene (3‐MC) to mice. Liver cell homogenates were subfractionated byExpand
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The Size of the Human Proteome: The Width and Depth
This work discusses bioinformatics and experimental approaches to explore the human proteome, a constellation of proteins expressed in different tissues and organs. As the human proteome is not aExpand
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Combination of virtual and experimental 2DE together with ESI LC‐MS/MS gives a clearer view about proteomes of human cells and plasma
Virtual and experimental 2DE coupled with ESI LC‐MS/MS was introduced to obtain better representation of the information about human proteome. The proteins from HEPG2 cells and human blood plasmaExpand
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Virtual-Experimental 2DE Approach in Chromosome-Centric Human Proteome Project.
To obtain more information about human proteome, especially about proteoforms (protein species) coded by 18th chromosome, we separated proteins from human cancer cell line (HepG2) by two-dimensionalExpand
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Human aqueous humor proteome in cataract, glaucoma, and pseudoexfoliation syndrome
Twenty‐nine human aqueous humor samples from patients with eye diseases such as cataract and glaucoma with and without pseudoexfoliation syndrome were characterized by LC–high resolution MS analysis.Expand
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Proteomics of mouse liver microsomes: Performance of different protein separation workflows for LC‐MS/MS
The mouse liver microsome proteome was investigated using ion trap MS combined with three separation workflows including SDS‐PAGE followed by reverse‐phase LC of in‐gel protein digestions (519Expand
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