• Publications
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Development and application of a polymerase chain reaction assay for Mycoplasma synoviae.
Mycoplasma synoviae (MS) species-specific primers selected from the 16S rRNA sequence were evaluated by polymerase chain reaction (PCR). The MS primers were MS-1 (5'-GAAGCAAAATAGTGATATCA-3') and MS-2Expand
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Rapid selection in chickens of subpopulations within ArkDPI-derived infectious bronchitis virus vaccines
We examined spike (S) gene sequences of the virus populations of four different commercial ArkDPI-derived infectious bronchitis coronavirus vaccines before and during a single passage in specificExpand
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Nucleotide sequence of an mRNA transcribed in latent growth-transforming virus infection indicates that it may encode a membrane protein.
The most abundant Epstein-Barr virus mRNA in a latently infected cell line, IB4, established by in vitro growth transformation with virus, was a 2,8-kilobase RNA encoded by largely unique DNA nearExpand
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Genetic characterization of chicken anemia virus from commercial broiler chickens in Alabama.
Chicken anemia virus (CAV) isolates show extremely limited genetic variability worldwide. We determined the nucleotide sequence of an 823-nucleotide portion of the 2.3-kb CAV genome found in 10 liverExpand
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Epidemiological and experimental evidence for immunodeficiency affecting avian infectious bronchitis
We evaluated the effects of viral immunodeficiency on the outcome of infectious bronchitis virus (IBV) infection in chickens as a hypothetical cause for failure of adequate protection in vaccinatedExpand
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Infectious Bronchitis Virus in the Chicken Harderian Gland and Lachrymal Fluid: Viral Load, Infectivity, Immune Cell Responses, and Effects of Viral Immunodeficiency
Abstract We compared detection of infectious bronchitis virus (IBV) by quantitative RT-PCR (qRT-PCR) in tears and trachea of IBV-infected chickens and found that quantitative detection of IBV RNA inExpand
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Analysis of bovine herpesvirus 4 genomic regions located outside the conserved gammaherpesvirus gene blocks.
Bovine herpesvirus 4 (BHV-4) DNA sequences located outside the gene blocks conserved among the gammaherpesviruses BHV-4, herpesvirus saimiri (HVS) and Epstein-Barr virus (EBV) were analysed. TwelveExpand
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Herpes Simplex Virus Type 1 ICP27 Regulates Expression of a Variant, Secreted Form of Glycoprotein C by an Intron Retention Mechanism
ABSTRACT We previously showed that herpes simplex virus type 1 (HSV-1) immediate-early (IE) protein ICP27 can posttranscriptionally stimulate mRNA accumulation from a transfected viral late geneExpand
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Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus.
Bovine herpesvirus-4 (BHV-4), a gammaherpesvirus lacking a clear disease association, productively infects multiple cell lines of various species and causes cell death. A human rhabdomyosarcoma cellExpand
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Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila.
A multiplex PCR (m-PCR) method was developed for simultaneous detection of 3 important fish pathogens in warm water aquaculture. The m-PCR to amplify target DNA fragments from FlavobacteriumExpand
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  • Open Access