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Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA
TLDR
The community structure of bacterioplankton in meromictic Lake Saelenvannet was examined by PCR amplification of the V3 region of 16S rRNA from microbial communities recovered from various depths in the water column, and bacterial diversity estimated from the number and intensity of specific fragments in DGGE profiles decreased with depth, while the reverse was true for the Archaea.
High diversity in DNA of soil bacteria
TLDR
The results show that the major part of DNA isolated from the bacterial fraction of soil is very heterogeneous, with a C0t1/2 value corresponding to about 4,000 completely different genomes of standard soil bacteria.
Microbial diversity and function in soil: from genes to ecosystems.
Prokaryotic Diversity--Magnitude, Dynamics, and Controlling Factors
There are probably millions of species in the microorganismal domains Bacteria and Archaea (the prokaryotes), and we are only just beginning to work out the basic principles governing their
Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products
Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis (DGGE) was tested as a means to study microbial community composition in hulk
Total bacterial diversity in soil and sediment communities—A review
TLDR
Reassociation analysis of DNA isolated directly from the bacteria in pristine soil and marine sediment samples revealed that such environments contained in the order of 10 000 bacterial types, which was approximately 170 times higher than the diversity of the collection of bacterial isolates from the same soil.
Pesticide effects on bacterial diversity in agricultural soils – a review
TLDR
Future research on pesticide approval protocols should establish the relationships between mineralisation assays and new and rapid bacterial diversity profiling methods, and should include the possible ecological implications of altered bacterial diversity for soil fertility.
Novel techniques for analysing microbial diversity in natural and perturbed environments.
TLDR
Using DNA reassociation analysis, it is demonstrated that bacterial communities in pristine soil and sediments may contain more than 10,000 different bacterial types, indicating that the culturing conditions select for a distinct subpopulation of the bacteria present in the environment.
Abundance and Diversity of Archaea in Heavy-Metal-Contaminated Soils
TLDR
Analysis of cloned 16S ribosomal DNA showed close similarities to a unique and globally distributed lineage of the kingdom Crenarchaeota that is phylogenetically distinct from currently characterized cren archaeotal species.
Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria
TLDR
This study showed that reassociation of DNA isolated from a collection of bacteria gave a good estimate of the diversity of the collection and that there was good agreement with different phenotypic diversity measures.
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