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Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
The community structure of bacterioplankton in meromictic Lake Saelenvannet was examined by PCR amplification of the V3 region of 16S rRNA from microbial communities recovered from various depths inExpand
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High diversity in DNA of soil bacteria.
Soil bacterium DNA was isolated by minor modifications of previously described methods. After purification on hydroxyapatite and precipitation with cetylpyridinium bromide, the DNA was sheared in aExpand
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Microbial diversity and function in soil: from genes to ecosystems.
Soils sustain an immense diversity of microbes, which, to a large extent, remains unexplored. A range of novel methods, most of which are based on rRNA and rDNA analyses, have uncovered part of theExpand
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Prokaryotic Diversity--Magnitude, Dynamics, and Controlling Factors
There are probably millions of species in the microorganismal domains Bacteria and Archaea (the prokaryotes), and we are only just beginning to work out the basic principles governing theirExpand
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Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products
Separation of polymerase chain reaction (PCR)-amplified 16S rDNA products using denaturing gradient gel electrophoresis (DGGE) was tested as a means to study microbial community composition in hulkExpand
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Total bacterial diversity in soil and sediment communities—A review
Advances in microbial methods have demonstrated that microorganisms globally are the dominating organisms both concerning biomass and diversity. Their functional and genetic potential may exceed thatExpand
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Pesticide effects on bacterial diversity in agricultural soils – a review
Abstract. According to guidelines for the approval of pesticides, side-effects on soil microorganisms should be determined by studying functional parameters such as carbon or nitrogen mineralisation.Expand
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Novel techniques for analysing microbial diversity in natural and perturbed environments.
Molecular techniques were applied for analysing the entire bacterial community, including both the cultivated and non-cultivated part of the community. DNA was extracted from samples of soils andExpand
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Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria.
The phenotypic diversity of about 200 bacterial strains isolated from soil was compared with the genotypic diversity of the same population. The strains were phenotypically characterized by the APIExpand
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Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms
This review mainly discusses three related topics: the application of ecological theories to soil, the measurement of microbial diversity by molecular techniques and the impact of transgenic plantsExpand
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