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S-Glutathiolation by peroxynitrite activates SERCA during arterial relaxation by nitric oxide
Nitric oxide (NO) physiologically stimulates the sarco/endoplasmic reticulum calcium (Ca2+) ATPase (SERCA) to decrease intracellular Ca2+ concentration and relax cardiac, skeletal and vascular smooth…
SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model.
- M. Harraz, J. Marden, J. Engelhardt
- Biology, ChemistryJournal of Clinical Investigation
- 1 February 2008
It is demonstrated that SOD1 is not just a catabolic enzyme, but can also directly regulate NADPH oxidase-dependent (Nox-dependent) O(2)(*-) production by binding Rac1 and inhibiting its GTPase activity.
Glutathione Peroxidase Protects against Peroxynitrite-mediated Oxidations
- H. Sies, V. Sharov, L. Klotz, K. Briviba
- Biology, ChemistryJournal of Biological Chemistry
- 31 October 1997
A novel function of GPx is demonstrated, and potentially of other selenoproteins containing selenocysteine or selenomethionine, in the GSH-dependent maintenance of a defense line against peroxynitrite-mediated oxidations, as a peroxlynitrite reductase.
3-Nitrotyrosine modification of SERCA2a in the aging heart: a distinct signature of the cellular redox environment.
It is shown that the additional loss of activity in the senescent Fischer 344 rat heart relative to that of young adult hearts is a result of increased 3-nitrotyrosine modification of the Ca-ATPase, and age-dependent increases in nitration of cardiac SERCA2a are identified using multiple analytical methods.
Diastereoselective reduction of protein‐bound methionine sulfoxide by methionine sulfoxide reductase
Anti-apoptotic protein Bcl-2 interacts with and destabilizes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA).
- E. Dremina, V. Sharov, KESHAVA N. Kumar, A. Zaidi, E. Michaelis, C. Schöneich
- Biology, Computer ScienceBiochemical Journal
- 15 October 2004
The results suggest that the direct interaction of Bcl-2 with SERCA may be involved in the regulation of apoptotic processes in vivo through modulation of cytoplasmic and/or endoplasmic reticulum calcium levels required for the execution of apoptosis.
Quantitative mapping of oxidation-sensitive cysteine residues in SERCA in vivo and in vitro by HPLC-electrospray-tandem MS: selective protein oxidation during biological aging.
- V. Sharov, E. Dremina, N. Galeva, T. Williams, C. Schöneich
- Biology, ChemistryBiochemical Journal
- 15 March 2006
A quantitative method to monitor the oxidation state of the individual SERCA cysteine residues using a maleimide-based fluorescence dye, TG1 (ThioGlo 1), as a label for cysteines that have not been altered by oxidation and are not involved in disulphide bridges is developed and optimized.
Oxidative inactivation of purified plasma membrane Ca2+-ATPase by hydrogen peroxide and protection by calmodulin.
- A. Zaidi, Lorena B. Barrón, V. Sharov, C. Schöneich, E. Michaelis, M. Michaelis
- Biology, ChemistryBiochemistry
- 21 October 2003
In the present studies, inhibition of PMCA by H( 2)O(2) was characterized in enzyme purified from human erythrocyte membranes, indicating a CaM-induced conformational state resistant to oxidation.
Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes
Overall, the data advocate for a Bcl-2-dependent mechanism of apoptosis in differentiated muscle cells, however, downstream processes for spontaneous and hydrogen peroxide induced apoptosis are not completely similar.