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High resolution structures of holo and apo formate dehydrogenase.
Three-dimensional crystal structures of holo (ternary complex enzyme-NAD-azide) and apo NAD-dependent dimeric formate dehydrogenase (FDH) from the methylotrophic bacterium Pseudomonas sp. 101 have
NAD(+)-dependent formate dehydrogenase.
TLDR
FDH is perhaps the most suitable model for investigating the general mechanism of catalysis involving hydride ion transfer, and is one of the most promising candidates for the development of so-called coenzyme regeneration systems.
Protein engineering of formate dehydrogenase.
Catalytic mechanism and application of formate dehydrogenase
NAD+-dependent formate dehydrogenase (FDH) is an abundant enzyme that plays an important role in energysupply of methylotrophic microorganisms and in response to stress in plants. FDH belongs to the
Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed*
TLDR
A study of the structure-function relationship of KillerRed has revealed the key residues most likely responsible for the phototoxic effect, and Glu68 and Ser119, located adjacent to the chromophore, have been assigned as the primary trigger of the reaction chain.
NAD-dependent formate dehydrogenase from methylotrophic bacterium, strain 1. Purification and characterization.
TLDR
The enzyme retained full activity upon storage at ambient temperature in solution for half a year in the presence of 2-mercaptoethanol or EDTA, and was rapidly inactivated in the absence of -SH compounds.
Structure‐guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+
TLDR
The results demonstrate that the combined polar interactions and steric factors comprise the main structural determinants responsible for coenzyme specificity.
Catalytic mechanism and application of formate dehydrogenase
TLDR
This review covers the late developments in cloning genes of FDH from various sources, studies of its catalytic mechanism and physiological role, and its application for new chiral syntheses.
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