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The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast.
The findings suggest that Ddc2 may be the functional homolog of Schizosaccharomyces pombe Rad26, strengthening the hypothesis that the mechanisms leading to checkpoint activation are conserved throughout evolution.
The novel DNA damage checkpoint protein Ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast
- M. Longhese, V. Paciotti, R. Fraschini, Raffaella Zaccarini, P. Plevani, G. Lucchini
- BiologyThe EMBO journal
- 1 September 1997
The DDC1 gene was identified, together with MEC3 and other checkpoint genes, during a screening for mutations causing synthetic lethality when combined with a conditional allele altering DNA primase, and is involved in all the known DNA damage checkpoints.
Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p
- V. Paciotti, G. Lucchini, P. Plevani, M. Longhese
- Biology, Computer ScienceThe EMBO journal
- 15 July 1998
It is suggested that Rad9p and Ddc1p might function in separated branches of the DNA damage checkpoint pathway, playing different roles in determining Mec1p activity and/or substrate specificity.
Characterization of mec1Kinase-Deficient Mutants and of New Hypomorphic mec1Alleles Impairing Subsets of the DNA Damage Response Pathway
- V. Paciotti, M. Clerici, M. Scotti, G. Lucchini, M. Longhese
- BiologyMolecular and Cellular Biology
- 15 June 2001
Two new hypomorphic mec1 mutants are described that are completely defective in the G1/S and intra-S DNA damage checkpoints but properly delay nuclear division after UV irradiation in G2.
The Plasticity of Dendritic Cell Responses to Pathogens and Their Components
The 70 kDa subunit of replication protein A is required for the G1/S and intra-S DNA damage checkpoints in budding yeast.
- M. Longhese, H. Neecke, V. Paciotti, G. Lucchini, P. Plevani
- BiologyNucleic acids research
- 1 September 1996
The hypersensitivity to UV and MMS treatments observed in the rfa1-M4 mutant might only be due to impairment of RPA function in DNA repair, while the rFA1- M2 mutation seems to affect both the DNA repair and checkpoint functions of Rpa70.
Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions
Interactions between SET1 and MEC3 have a role in DNA repair and telomere function, and restoration of TPE in a set1Δ mutant by overexpression of the isolated SET domain requires Mec3p.
Checkpoint proteins influence telomeric silencing and length maintenance in budding yeast.
The finding that telomere shortening, but not increased telomeric repression of gene expression in rad53 mutants, can be suppressed by increasing dNTP synthetic capacity in these strains suggests that transcriptional silencing and telomeres integrity involve separable functions of Rad53.
Hyperactivation of the yeast DNA damage checkpoint by TEL1 and DDC2 overexpression
- M. Clerici, V. Paciotti, V. Baldo, M. Romano, G. Lucchini, M. Longhese
- BiologyThe EMBO journal
- 15 November 2001
It is shown that regulation of Tel1 and Ddc2–Mec1 activities is important to modulate both activation and termination of checkpoint‐mediated cell cycle arrest, and that unscheduled Rad53 phosphorylation might prevent cells from re‐entering the cell cycle after checkpoint activation.
A role for DNA primase in coupling DNA replication to DNA damage response
The results suggest that DNA primase plays an essential role in a subset of the Rad53p‐dependent checkpoint pathways controlling cell cycle progression in response to DNA damage.