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Quantitative Classification of Somatostatin-Positive Neocortical Interneurons Identifies Three Interneuron Subtypes
Deciphering the circuitry of the neocortex requires knowledge of its components, making a systematic classification of neocortical neurons necessary. GABAergic interneurons contribute most of theExpand
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Two-photon photostimulation and imaging of neural circuits
We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This methodExpand
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SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators
Laser microscopy has generally poor temporal resolution, caused by the serial scanning of each pixel. This is a significant problem for imaging or optically manipulating neural circuits, sinceExpand
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Sodium channels amplify spine potentials
Dendritic spines mediate most excitatory synapses in the brain. Past theoretical work and recent experimental evidence have suggested that spines could contain sodium channels. We tested this byExpand
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Multiphoton stimulation of neurons.
We pulsed the activation of neurons using a femtosecond laser. Pyramidal neurons are depolarized and fire action potentials when high intensity mode-locked infrared light irradiates somatic membranesExpand
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A two-photon and second-harmonic microscope.
Two-photon microscopy has revolutionized life sciences by enabling long-term imaging of living preparations in highly scattering tissue while minimizing photodamage. At the same time, commercialExpand
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Second harmonic imaging of membrane potential of neurons with retinal.
We present a method to optically measure and image the membrane potential of neurons, using the nonlinear optical phenomenon of second harmonic generation (SHG) with a photopigment retinal as theExpand
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Two-photon uncaging of neurochemicals using inorganic metal complexes.
Neuroactive compounds can be photoreleased by means of two-photon excitation using a new kind of transition metal-based caged compound.
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A portable laser photostimulation and imaging microscope.
TLDR
We describe a compact microscope that uses a spatial light modulator (SLM) to control the excitation laser light and also perform functional imaging of neuronal activity in living brain slices. Expand
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How to build a two-photon microscope with a confocal scan head.
This article provides practical guidelines for the conversion of a standard confocal microscope into a two-photon microscope. This conversion enables the investigator to have access to two-photonExpand
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