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A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery
TLDR
A versatile platform for the isolation of peptides delivering a wide range of cargos into the cytoplasm of cells, and establishes a discovery platform for identifying novel, functional CPPs to expand the delivery landscape of druggable intracellular targets for biological therapeutics.
PASylation of Murine Leptin Leads to Extended Plasma Half-Life and Enhanced in Vivo Efficacy.
TLDR
PASylated leptin not only provides a promising reagent to study its physiological role in vivo but also may offer a superior drug candidate for clinical therapy.
The in vitro PIG-A gene mutation assay: glycosylphosphatidylinositol (GPI)-related genotype-to-phenotype relationship in TK6 cells
TLDR
This work investigated the GPI-related genotype-to-phenotype relationship in TK6 cells and identified a heterozygous chromosomal deletion on chromosome 17, where the PIG-L gene is located, which could be responsible for the increased sensitivity toward mutagenic ethyl methanesulfonate or UV-C treatments of p53-proficient TK8 cells.
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma
TLDR
Cereblon is identified as a co-chaperone that specifically determines chaperone activity of HSP90 toward transmembrane proteins by means of counteracting AHA1, establishing the CRBN-AHA1-HSP90 axis in the biogenesis of transmemBRane proteins.
Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy
TLDR
This work fusing rAAV-2 capsids with two modular targeting molecules specific for a cancer cell-surface marker (EGFR) while simultaneously including an affinity tag (His-tag) in a surface-exposed loop to demonstrate tumor marker specific transduction and prodrug-dependent apoptosis of cancer cells.
Selection and characterization of Anticalins targeting human prostate-specific membrane antigen (PSMA).
TLDR
Anticalins resulting from this study offer a viable alternative to antibody-based PSMA binders for biomedical applications, including in vivo imaging of PCa or neovasculature of solid tumors.
Novel Monoclonal Antibodies Recognizing Human Prostate‐Specific Membrane Antigen (PSMA) as Research and Theranostic Tools
TLDR
The detailed characterization of four novel murine monoclonal antibodies recognizing human PSMA as well as PSMA orthologs from different species is reported.
Long-Acting PASylated Leptin Ameliorates Obesity by Promoting Satiety and Preventing Hypometabolism in Leptin-Deficient Lep(ob/ob) Mice.
TLDR
The beneficial properties of PAS(600)-leptin are substantiated by a comparison with previous studies in which approximately 400 nmol (∼25-fold) unmodified leptin was mandatory to achieve similar improvements.
Treatment of diet-induced lipodystrophic C57BL/6J mice with long-acting PASylated leptin normalises insulin sensitivity and hepatic steatosis by promoting lipid utilisation
TLDR
The excellent pharmacological properties render PASylated leptin an agent of choice for refining both animal studies and therapeutic strategies in the context of LD syndromes and beyond.
PASylated interferon &agr; efficiently suppresses hepatitis B virus and induces anti‐HBs seroconversion in HBV‐transgenic mice
TLDR
PASylated IFN&agr; showed a profoundly increased antiviral effect in vivo compared to the non‐modified version without toxicity, providing proof‐of‐concept that an improved IFN-AGr; can achieve higher rates of HBV antiviral and immune control.
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