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Induction of apoptosis in uninfected lymphocytes by HIV-1 Tat protein.
Infection by human immunodeficiency virus-type 1 (HIV-1) is typified by the progressive depletion of CD4 T lymphocytes and deterioration of immune function in most patients. A central unresolvedExpand
Inhibition of Plasmodium falciparum malaria using antisense oligodeoxynucleotides.
We studied inhibition of growth of the malaria parasite Plasmodium falciparum in in vitro culture using antisense (AS) oligodeoxynucleotides (ODNs) against different target genes. W2 and W2mefExpand
Antimycobacterial activities of antisense oligodeoxynucleotide phosphorothioates in drug-resistant strains.
Strains of Mycobacterium smegmatis, a mycobacterium which shares genetic sequences, grows more rapidly, and is nonpathogenic in man as compared with Mycobacterium tuberculosis, were utilized for theExpand
Plasmodium falciparum: effect of chemical structure on efficacy and specificity of antisense oligonucleotides against malaria in vitro.
Antisense oligodeoxynucleotides (AS ODNs) have shown promise both as potential anti-malarial chemotherapeutic agents and as a means for identifying genes critical for parasite survival. BecauseExpand
PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic
We present here the first detailed biochemical analysis of an archaeal restriction enzyme. PspGI shows sequence similarity to SsoII, EcoRII, NgoMIV and Cfr10I, which recognize related DNA sequences.Expand
Site‐specific enzymatic cleavage of TMV RNA directed by deoxyribo‐ and chimeric (deoxyribo‐ribo)oligonucleotides
The TMV RNA molecule can be cleaved at a single site by RNase H directed by chimeric oligo(deoxyribo‐ribo)nucleotide with an internucleotide pyrophosphate bond
Regulation of activity of transcription factor NF-κB by synthetic oligonucleotides
Eukaryotic dimeric nuclear factor-κB (NF-κB) is one of the main transcription factors that activate expression of genes, products of which play the key role in development of cardiovascularExpand
Specific conjugation of DNA binding proteins to DNA templates through thiol–disulfide exchange
The double‐stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficientExpand
DNA-methyltransferase SsoII as a bifunctional protein: Features of the interaction with the promoter region of SsoII restriction-modification genes
DNA duplexes bearing an aldehyde group at the 2′-position of the sugar moiety were used for affinity modification of (cytosine-5)-DNA methyltransferase SsoII. It is shown that lysine residues ofExpand
Structure‐based cross‐linking of NF‐κB p50 homodimer and decoy bearing a novel 2'‐disulfide trapping site
Double‐stranded oligodeoxyribonucleotides with engineered disulfide units were successfully used for covalent trapping of cysteine containing proteins. In particular, an efficient cross‐linking ofExpand